Cyanobacteria contain a mitochrondrial complex I‐homologous NADH‐dehydrogenase
1991; Wiley; Volume: 286; Issue: 1-2 Linguagem: Inglês
10.1016/0014-5793(91)80957-5
ISSN1873-3468
AutoresSusanne Berger, Ulrike Ellersiek, Klaus Steinmüller,
Tópico(s)ATP Synthase and ATPases Research
ResumoThylakoid and cytoplasmic membranes of the cyanobacterium Synechocystis sp. PCC 6803 were purified by sucrose gradient centrifugation. Both membranes oxidize NADH in a rotenone‐sensitive reaction. Antibodies prepared against psbG/ndhK and ndhJ fusion proteins detect the corresponding polypeptides in both membrane preparations. This demonstrates that a NADH‐dehydrogenase, homologous to the mitochondrial NADH‐ubiquinone‐oxidoreductase (complex I of the respiratory chain) is present in cyanobacteria. The NADH‐dehydrogenase can be solubilized with the detergent β‐D‐dodecylmaltoside. Sedimentation analysis of the solubilized enzyme on a sucrose gradient indicates that it is a multisubunit protein complex.
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