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Nocodazole inhibition of the vasopressin-induced water permeability increase in toad urinary bladder

1981; Elsevier BV; Volume: 646; Issue: 3 Linguagem: Inglês

10.1016/0005-2736(81)90308-4

1879-2642

Robert J. Brady, Robert H. Parsons, Lynne M. Coluccio,

Circadian rhythm and melatonin

Nocodazole is a synthetic antitumor drug that binds rapidly to tubulin. When this drug is applied to toad bladder prior to vasopressin stimulation it inhibits the vasopressin response. A maximum inhibition (68%) is reached with a dose level of 10 μ/ml applied one-half hour prior to vasopressin stimulation (20 mU/ml). This compares with an inhibition of 50% seen with a 3-h exposure of the tissue to colchicine (0.1 mM) prior to stimulation with vasopressin. Application of nocodazole (1 μ/ml) 3 min after hormonal stimulation shows no inhibition of the response at one-half hour past stimulation. These data support the view that microtubules are involved in the vasopressin-induced increase in water permeability in toad bladder and also indicate that this involvement is limited to the period prior to or directly after stimulation.