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Intact Genetic Material is Present in Commercially Processed Cadaver Allografts Used for Pubovaginal Slings

2002; Lippincott Williams & Wilkins; Volume: 168; Issue: 3 Linguagem: Inglês

10.1016/s0022-5347(05)64570-3

1527-3792

Jon K. Hathaway, JONG M. CHOE,

Tissue Engineering and Regenerative Medicine

No AccessJournal of UrologyCLINICAL UROLOGY: ORIGINAL ARTICLES1 Sep 2002Intact Genetic Material is Present in Commercially Processed Cadaver Allografts Used for Pubovaginal Slings Jon K. Hathaway and Jong M. Choe Jon K. HathawayJon K. Hathaway More articles by this author and Jong M. ChoeJong M. Choe More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)64570-3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We determined the presence, quantitated the concentration and assessed the length of DNA present in 4 commercially available human cadaver allografts. Materials and Methods: We evaluated 10 tissue samples from each of 4 commercial sources of human allograft (Mentor Corp., Santa Barbara, California; Musculoskeletal Transplant Foundation, Edison, New Jersey; Regeneration Technologies, Inc., Alachua, Florida; and Life Cell Corp., Woodlands, Texas) for intact DNA segments. All allograft samples underwent a standard extraction technique (proteinase K/sodium dodecyl sulfate/phenol) to isolate genetic material. Spectrophotometry evaluation was done to quantify DNA concentrations. Polymerase chain reaction was performed to amplify the retrieved DNA material. Agarose gel electrophoresis was performed to determine the size of DNA fragments. Results: Of the 49 samples tested from all 4 commercial sources of human allograft fascia 39 (97.5%) contained DNA of 400 to 2,000 bp segments. A 400 bp DNA segment was present in 9 Mentor, 10 Musculoskeletal Transplant Foundation, 10 Regeneration Technologies and 10 Life Cell samples. A 700 bp DNA segment was present in 10 Musculoskeletal Transplant Foundation, 10 Regeneration Technologies and 10 Life Cell allografts. A 2,000 bp DNA segment was present in 10 Life Cell tissues. Conclusions: Intact genetic material was present in all 4 commercially processed human allografts. Tissue processing did not completely eliminate intact DNA segments. The size of the intact DNA and the concentration of DNA varied widely based on tissue processing methods. References 1 : Cadaver donor screening for infectious agents in solid organ transplantation. Clin Infect Dis2000; 31: 781. Crossref, Medline, Google Scholar 2 : HIV transmission by organ tissue transplantation. AIDS1993; 7: S35. Crossref, Medline, Google Scholar 3 : Bone transplantation human immunodeficiency virus. An estimate of risk of acquired immunodeficiency syndrome (AIDS). Clin Orthop1989; 240: 129. Crossref, Google Scholar 4 : Risk of virus transmission by tissue, blood, plasma products. 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LATINI J, LUX M and KREDER K (2018) Efficacy and Morbidity of Autologous Fascia Lata Sling CystourethropexyJournal of Urology, VOL. 171, NO. 3, (1180-1184), Online publication date: 1-Mar-2004.LATINI J, BROWN J and KREDER K (2018) Abdominal Sacral Colpopexy Using Autologous Fascia LataJournal of Urology, VOL. 171, NO. 3, (1176-1179), Online publication date: 1-Mar-2004. Volume 168Issue 3September 2002Page: 1040-1043 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordscadavertransplantation, homologousgenesbladderdisease transmissionMetricsAuthor Information Jon K. Hathaway More articles by this author Jong M. Choe More articles by this author Expand All Advertisement PDF downloadLoading ...