On the problem of dehydration and intracellular crystallization during freezing of cell suspensions
1976; Elsevier BV; Volume: 13; Issue: 2 Linguagem: Inglês
10.1016/0011-2240(76)90126-7
ISSN1090-2392
AutoresN.S. Pushkar, Y.A. Itkin, V.L. Bronstein, E.A. Gordiyenko, Y.V. Kozmin,
Tópico(s)Freezing and Crystallization Processes
ResumoThe kinetic equation of the process of cell dehydration during freezing has been obtained. It is used to assess the degree of protoplasmic supercooling as a function of the cooling rate and cell parameters. The suggested model of dehydration cannot be applied to cells with permeability coefficients for water molecules more than 10−5 cm/sec · bar, in particular to erythrocytes. The peculiarities of intracellular crystallization in red cells have been studied. The results show that red cells are likely to start freezing at cooling rates slower than those supposed from calculations of Mazur (9).
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