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Magnetic polymeric microspheres for protein adsorption

2005; Elsevier BV; Volume: 236; Issue: 1-4 Linguagem: Inglês

10.1016/j.nimb.2005.04.056

1872-9584

M.C.F.C. Felinto, Duclerc Fernandes Parra, Ademar B. Lugão, Marina Batista, Olga Z. Higa, M. Yamaura, R.L. Camilo, M.T.C.P. Ribela, L. C. Sampaio,

Iron oxide chemistry and applications

Magnetic beads consisting of polymer-coated manganese ferrite nanoparticles were prepared by the precipitation reaction of manganese ferrite into the channels of methyl methacrylate polymer beads by sodium hydroxide, resulting in MnMagBead. MnMagBead was characterized by infrared spectra (FTIR), thermogravimetric analysis of TGA/DTG and indicates the presence of –CO (carbonyl) groups and the MnFe2O4 on the beads. Magnetization measurements were obtained at room temperature in magnetic fields up to 10 KOe using a vibrating sample magnetometer. Introductory Protein adsorption biological tests were processed using labeled I-125 albumin (BSA), and the activity was measured in a gamma counting spectrometer. These superparamagnetic beads exhibit the capacity to bind biological molecules such as proteins like albumin, with a good capability (5 × 10−6) μg/100 mg of beads as compared with other magnetic resins studied in our group.