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Uneven distribution of intracellular Cl− in rat hippocampal neurons

1992; Elsevier BV; Volume: 143; Issue: 1-2 Linguagem: Inglês

10.1016/0304-3940(92)90250-b

1872-7972

Mitsuyoshi Hara, Masafumi Inoue, Tohru Yasukura, Sumio Ohnishi, Yoshito Mikami, Chiyoko Inagaki,

Neuroscience and Neuropharmacology Research

Electrophysiological observations of neurons suggest that perikarya and dendrites differ in local intracellular Cl- concentration ([Cl-]i), that has not been demonstrated yet. Regional [Cl-]i in cultured hippocampal neurons was estimated using a Cl(-)-sensitive fluorescent dye. Calibration showed that perikaryonic [Cl-]i was lower than dendritic [Cl-]i. Ethacrynic acid, an inhibitor of the outwardly directed Cl(-)-pump, increased the perikaryonic but not dendritic [Cl-]i. A decrease in [Cl-]i induced by furosemide or bumetanide, inhibitors of Na+/K+/2Cl- cotransporters, was more prominent in dendrites than in perikarya. These findings suggest that uneven distribution of Cl- is generated by the region-specific localization of these transporters.