Peroxynitrite Diminishes Gap Junctional Communication: Protection by Selenite Supplementation
1999; Wiley; Volume: 48; Issue: 4 Linguagem: Inglês
10.1080/713803538
ISSN1521-6551
AutoresVictor S. Sharov, Karlis Briviba, Helmut Sies,
Tópico(s)Glutathione Transferases and Polymorphisms
ResumoAbstract Loss of intercellular communication via gap junctions has been correlated with progression of cells to a malignant phenotype. Here, we show that peroxynitrite, a mediator of toxicity in inflammatory processes, diminishes gap junctional intercellular communication (GJIC) in WB‐F344 rat liver epithelial cells, assayed by the scrapeloading dye‐transfer technique as well as by microinjection of a fluorescent dye into single cells. Exposure of cultured cells to a steady‐state concentration of peroxynitrite of 1.6 muM for 4 min or to 3‐morpholinosydnonimine (SIN‐1) at 0.5 mM strongly diminished GJIC. These concentrations of peroxynitrite or SIN‐1 were not cytotoxic. When cells were grown in a medium supplemented with sodium selenite (0.1‐1 muM) for 72 h, substantial protection was afforded against the decrease in GJIC by peroxynitrite. Thus, peroxynitrite can disrupt GJIC, and selenium‐containing proteins protect.
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