A Novel Hydrogen Peroxide Biosensor Based on the Synergistic Effect of Gold‐Platinum Alloy Nanoparticles/Polyaniline Nanotube/Chitosan Nanocomposite Membrane
2009; Wiley; Volume: 21; Issue: 7 Linguagem: Inglês
10.1002/elan.200804479
ISSN1521-4109
AutoresXueliang Wang, Tao Yang, Yuanyuan Feng, Kui Jiao, Guicun Li,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoAbstract Based on the immobilization of horseradish peroxidase (HRP) in chitosan(CS) on a glassy carbon electrode (GCE) modified with the Au‐Pt alloy nanoparticles (NPs) / polyaniline nanotube (nanoPAN) nanocomposite film, a novel hydrogen peroxide biosensor was constructed. The modified processes of GCE were monitored by cyclic voltammetry and electrochemical impedance spectroscopy. Au‐PtNPs/nanoPAN/CS had a better synergistic electrochemical effect than did AuNPs/nanoPAN/CS or PtNPs/nanoPAN/CS. The amperometric response of the biosensor towards H 2 O 2 was investigated by successively adding aliquots of H 2 O 2 to a continuous stirring phosphate buffer solution under the optimized conditions. Because Au‐PtNPs have unique catalytic properties and good biocompatibility, and especially Au‐PtNPs and nanoPAN have synergistic augmentation for facilitating electron‐transfer, the biosensor displayed a fast response time (<2 s) and broad linear response to H 2 O 2 in the range from 1.0 to 2200 μmol L −1 with a relatively low detection limit of 0.5 μmol L −1 at 3 times the background noise. Moreover, the biosensor can be applied in practical analysis and exhibited high sensitivity, good reproducibility, and long‐term stability.
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