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Cyclic-strain-induced endothelial cell expression of adhesion molecules and their roles in monocyte-endothelial interaction

1999; Wiley; Volume: 44; Issue: 1 Linguagem: Inglês

10.1002/(sici)1097-4636(199901)44

1097-4636

Jong K. Yun, James M. Anderson, Nicholas P. Ziats,

Coronary Interventions and Diagnostics

Vascular endothelial cells (ECs) are constantly subjected to hemodynamic forces that may regulate monocyte–endothelial interaction in vivo. To examine the effects of cyclic strain on endothelial expression of monocyte adhesion molecules, E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) ECs were exposed to physiologically relevant levels of cyclic strain. When ECs were under 25% maximal strain at 30 cycles/min for 24 h, the expression of E-selectin significantly (p < 0.05) increased, by 83%, compared to control ECs under static conditions. Similarly, monocyte adhesion to ECs under strain (maximum of 15 or 25% at 30 and 60 cycles/min for 24 h) also significantly (p < 0.05) increased, by >82%. This cyclic-strain-induced monocyte adhesion was substantially inhibited (83.5%) by anti-E-selectin antibody. ICAM-1 expression also significantly increased, by 62%, when ECs were under 25% maximal strain at 30 cycles/min for 3 h whereas VCAM-1 expression by ECs under strain (for 0.5, 3, and 24 h) did not change compared to static ECs. When ECs were treated with anti-ICAM-1 antibody and monocytes with anti-VLA-4 antibody, an increase in monocyte adhesion to ECs under cyclic strain was reduced significantly. These results demonstrate that cyclic strain can induce EC expression of monocyte adhesion molecules E-selectin, ICAM-1, and VCAM-1 in a time-dependent manner and thus can mediate monocyte adhesion. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 44, 87–97, 1999.