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Electrocatalytic reduction of organic peroxides in organic solvents by microperoxidase-11 immobilized as a monolayer on a gold electrode

1996; Elsevier BV; Volume: 417; Issue: 1-2 Linguagem: Inglês

10.1016/s0022-0728(96)04736-5

1873-2569

Andrew N. J. Moore, Eugenii Katz, Itamar Willner,

Analytical Chemistry and Sensors

The heme-containing undecapeptide microperoxidase-11 was immobilized as a monolayer on gold wire electrodes by carbodiimide coupling of the peptide to a cystamine monolayer. Direct communication between the electrode and the heme center was observed in acetonitrile and ethanol. The observed formal potential for the FeIII/FeII couple for the immobilized microperoxidase-11 was −0.290 V vs. SCE in acetonitrile and −0.286 V in ethanol. These electrodes were applied as amperometric sensors for organic peroxides in acetonitrile and ethanol. The electrocatalytic reduction of cumene hydroperoxide, t-butyl hydroperoxide and hydrogen peroxide was demonstrated. In acetonitrile, kinetic parameters (Km and Imax) were evaluated using a modified Michaelis-Menten analysis for the electrocatalysis of the reduction of cumene hydroperoxide, t-butyl hydroperoxide and hydrogen peroxide.