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Fluorescence immunohistochemical detection of hypoxic cells in spheroids and tumours

1987; Springer Nature; Volume: 56; Issue: 4 Linguagem: Inglês

10.1038/bjc.1987.213

1532-1827

J A Raleigh, G.G. Miller, AJ Franko, CJ Koch, AF Fuciarelli, DA Kelly,

Photodynamic Therapy Research Studies

Polyclonal antibodies have been raised in rabbits to a haemocyanin adduct of a reductively-activated, fluorinated analogue of misonidazole. Fluorescence immunohistochemical studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in patterns similar to those revealed by autoradiographic studies with a tritium-labelled derivative of the fluorinated misonidazole analogue.