The MEK Pathway Is Required for Stimulation of p21WAF1/CIP1 by Transforming Growth Factor-β
1999; Elsevier BV; Volume: 274; Issue: 50 Linguagem: Inglês
10.1074/jbc.274.50.35381
ISSN1083-351X
AutoresPatrick Hu, Xing Shen, David Huang, Yueyi Liu, Christopher M. Counter, Xiao-Fan Wang,
Tópico(s)Hedgehog Signaling Pathway Studies
ResumoTransforming growth factor-β (TGF-β)can induce the cyclin-dependent kinase inhibitors p21 and p15 in a variety of cell types. We have shown previously that Smad3 is required for the growth inhibitory activity of TGF-β, whereas overexpression of Smads is not sufficient to activate the expression of p21 in HaCaT cells. These data suggest that an additional signaling pathway may be involved in stimulating p21 in HaCaT cells. Given the recent finding that the mitogen-activated protein kinase (MAPK) pathway can cause p21 induction and arrest cells, we examined the involvement of this pathway for p21 and p15 induction by TGF-β. We found that TGF-β can regulate the MAPK pathway, leading to the increased transactivation ability of transcription factor Elk. Constitutively active components in the MAPK pathway activate p21 expression, and inhibitors or dominant negative constructs for the MAPK pathway significantly decrease p21 induction by TGF-β. Both constitutively active MEK and inhibitors for MEK have no effect on Smad activity, including DNA binding, localization, and interaction with coactivator p300/CBP. These findings suggest that the MAPK pathway may be an independent pathway that is involved in p21 and p15 induction by TGF-β. Transforming growth factor-β (TGF-β)can induce the cyclin-dependent kinase inhibitors p21 and p15 in a variety of cell types. We have shown previously that Smad3 is required for the growth inhibitory activity of TGF-β, whereas overexpression of Smads is not sufficient to activate the expression of p21 in HaCaT cells. These data suggest that an additional signaling pathway may be involved in stimulating p21 in HaCaT cells. Given the recent finding that the mitogen-activated protein kinase (MAPK) pathway can cause p21 induction and arrest cells, we examined the involvement of this pathway for p21 and p15 induction by TGF-β. We found that TGF-β can regulate the MAPK pathway, leading to the increased transactivation ability of transcription factor Elk. Constitutively active components in the MAPK pathway activate p21 expression, and inhibitors or dominant negative constructs for the MAPK pathway significantly decrease p21 induction by TGF-β. Both constitutively active MEK and inhibitors for MEK have no effect on Smad activity, including DNA binding, localization, and interaction with coactivator p300/CBP. These findings suggest that the MAPK pathway may be an independent pathway that is involved in p21 and p15 induction by TGF-β. transforming growth factor-β mitogen-activated protein kinase glutathione S-transferase polyacrylamide gel electrophoresis Transforming growth factor-β is a cytokine that causes growth arrest in late G1 of the cell cycle (reviewed in Refs. 1Derynck R. Feng X.H. Biochim. Biophys. Acta. 1997; 1333: 105-150Crossref PubMed Scopus (508) Google Scholarand 2Hu P.P.-C. Datto M.B. Wang X.-F. Endocr. Rev. 1998; 19: 349-363Crossref PubMed Google Scholar). In the human keratinocyte cell line HaCaT, growth inhibition is thought to depend on the ability of TGF-β1 to increase synthesis of the cyclin-dependent kinase inhibitors p21 and p15. Increased p21 leads to an increase in its association with cyclin D-CDK4 and cyclin E-CDK2 and a decrease in cyclin-CDK complex activity. Increased p15 expression leads to an increase in association and sequestration of CDK4 and CDK6 from the regulatory cyclin partners. On the cell membrane, TGF-β ligand binds to the type II receptor, which recruits the type I receptor into the complex. The type I receptor is phosphorylated by the type II receptor kinase and, when activated, phosphorylates Smad2 and Smad3, allowing them to heteromerize with Smad4 and translocate into the nucleus. In the nucleus, Smad3 can bind to DNA with the consensus sequence GTCTAGAC that is found in the promoters of many TGF-β-regulated genes (3Zawel L. Dai J.L. Buckhaults P. Zhou S. Kinzler K.W. Vogelstein B. Kern S.E. Mol. Cell. 1998; 1: 611-617Abstract Full Text Full Text PDF PubMed Scopus (891) Google Scholar, 4Dennler S. Itoh S. Vivien D. ten Dijke P. Huet S. Gauthier J.M. EMBO J. 1998; 17: 3091-3100Crossref PubMed Scopus (1588) Google Scholar, 5Yingling J.M. Datto M.B. Wong C. Frederick J.P. Liberati N.T. Wang X.-F. Mol. Cell. Biol. 1997; 17: 7019-7028Crossref PubMed Google Scholar, 6Jonk L.J.C. Itoh S. Heldin C.-H. ten Dijke P. Kruijer W. J. Biol. Chem. 1998; 273: 21145-21152Abstract Full Text Full Text PDF PubMed Scopus (513) Google Scholar, 7Vindevoghel L. Kon A. Lechleider R.J. Uitto J. Roberts A.B. Mauviel A. J. Biol. Chem. 1998; 273: 13053-13057Abstract Full Text Full Text PDF PubMed Scopus (101) Google Scholar, 8Wong C. Rougier-Chapman E. Frederick J. Datto M. Liberati N. Li J.-M. Wang X.-F. Mol. Cell. Biol. 1999; 19: 1821-1830Crossref PubMed Scopus (230) Google Scholar), as well as to other proteins such as the transcriptional coactivators p300 and CBP (9Nishihara A. Hanai J.I. Okamoto N. Yanagisawa J. Kato S. Miyazono K. Kawabata M. Genes Cells. 1998; 3: 613-623Crossref PubMed Scopus (130) Google Scholar, 10Shen X. Hu P.P.-C. Liberati N.T. Datto M.B. Frederick J.P. Wang X.-F. Mol. Biol. Cell. 1998; 9: 3309-3319Crossref PubMed Scopus (185) Google Scholar, 11Janknecht R. Wells N.J. Hunter T. Genes Dev. 1998; 12: 2114-2119Crossref PubMed Scopus (437) Google Scholar, 12Feng X.-H. Zhang Y. Wu R.-Y. Derynck R. Genes Dev. 1998; 12: 2153-2163Crossref PubMed Scopus (451) Google Scholar) in a phosphorylation-dependent manner. Smad3 is also required for TGF-β-mediated growth inhibition because murine embryonic fibroblasts deficient in Smad3 have lost their ability to become growth inhibited by TGF-β (13Datto M.B. Frederick J.P. Pan L. Borton A.J. Zhuang Y. Wang X.F. Mol. Cell. Biol. 1999; 19: 2495-2504Crossref PubMed Google Scholar). Although Smads are required for TGF-β-mediated growth arrest, the mechanism underlying their action is still unknown. In HaCaT cells, TGF-β induction of the cyclin-dependent kinase inhibitors p21 and p15 correlates with both Smad nuclear translocation as well as growth arrest, but when Smad3 and Smad4 are overexpressed, they are unable to activate p21 and p15 transcription. In contrast, overexpression of Smad3 and Smad4 is sufficient to activate transcription of plasminogen activator inhibitor (14Zhang Y. Feng X.-H. Wu R.-Y. Derynck R. Nature. 1996; 383: 168-172Crossref PubMed Scopus (759) Google Scholar), which contains Smad-binding elements in its promoter region (4Dennler S. Itoh S. Vivien D. ten Dijke P. Huet S. Gauthier J.M. EMBO J. 1998; 17: 3091-3100Crossref PubMed Scopus (1588) Google Scholar, 15Stroschein S.L. Wang W. Luo K. J. Biol. Chem. 1999; 274: 9431-9441Abstract Full Text Full Text PDF PubMed Scopus (110) Google Scholar). This suggests that even though Smads may be required for growth inhibition by TGF-β, they are not sufficient to drive the transcription of p21 and p15, indicating that other pathways may also be needed for the transcriptional induction of p21 and p15 upon TGF-β treatment. Contrary to their role as the mediators of many mitogens, the MAPKs have been recently implicated in cellular growth arrest and senescence. Overexpression of Raf leads to growth arrest by impinging directly on the cell cycle through increasing p21 expression, leading to its association and concomitant inhibition of CDKs (16Lloyd A.C. Obermuller F. Staddon S. Barth C.F. McMahon M. Land H. Genes Dev. 1997; 11: 663-677Crossref PubMed Scopus (206) Google Scholar, 17Woods D. Parry D. Cherwinski H. Bosch E. Lees E. McMahon M. Mol. Cell. Biol. 1997; 17: 5598-5611Crossref PubMed Scopus (577) Google Scholar). In addition, altering the cellular ratio of existing Ras and Rho proteins can also lead to stimulation of p21 (18Olson M.F. Paterson H.F. Marshall C.J. Nature. 1998; 394: 295-299Crossref PubMed Scopus (410) Google Scholar). TGF-β can also increase GTP-bound Ras, and activate MAPK pathway in other cell types (19Mulder K.M. J. Biol. Chem. 1992; 267: 5029-5031Abstract Full Text PDF PubMed Google Scholar). Because activation of the Ras-Raf-MEK-MAPK pathway can induce transcription of p21, in this study we asked whether this pathway was involved in the ability of TGF-β to stimulate p21 and p15 expression. Here, we show that overexpression of constitutively active forms of different components in the MAPK cascade can substitute for the ability of TGF-β to stimulate p15 and p21 reporter activity. Stable lines overexpressing a constitutively active form of MEK, MEK Q56P (20Lin A.W. Barradas M. Stone J.C. van Aelst L. Serrano M. Lowe S.W. Genes Dev. 1998; 12: 3008-3019Crossref PubMed Scopus (771) Google Scholar), also have increased expression of p21. Furthermore, inhibitors of this pathway prevent TGF-β induction of p21 and reduce TGF-β-mediated growth arrest in parental HaCaT cells. In contrast, TGF-β activation of Smad signaling is not affected by these inhibitors. Taken together, these data suggest that the MAPK pathway is involved in p21 up-regulation by TGF-β. The human p21 and p15 minimal promoter constructs, p21P93S and p15P113S, have been described previously (21Datto M.B. Yu Y. Wang X.-F. J. Biol. Chem. 1995; 270: 28623-28628Abstract Full Text Full Text PDF PubMed Scopus (399) Google Scholar). GAL4-Sp1 and GAL4-Sp3 full-length, GAL4-Sp1, and Sp3 B domain constructs have been were described previously (22Li J.M. Datto M.B. Shen X. Hu P.P. Yu Y. Wang X.F. Nucleic Acids Res. 1998; 26: 2449-2456Crossref PubMed Scopus (92) Google Scholar). GAL4-Elk, Ras 17A, Raf-N4, Raf-CAAX, MEK S218/222D, Rac 17N, cdc42 17N, MKK4/SEK-1, and SEK-AL constructs were the generous gift of Dr. Channing Der. The MEK Q56P pBABE-puro construct was the generous gift of Dr. Scott Lowe. HaCaT cells were the gift of Dr. Baukamp and Dr. Fusenig. HaCaT were maintained in 10% fetal bovine serum (Life Technologies, Inc) in α-minimum essential medium (Life Technologies, Inc.). For luciferase assays, cells were lysed, and luciferase activity in the lysates were assayed by integrating total light emission over 30 s using a Berthold luminometer. The luciferase activities were normalized based on the amount of expressed β-galactosidase activity expressed from co-transfected pCMV-β-galactosidase. Cells were lysed in universal lysis buffer (ULB) as described previously (10Shen X. Hu P.P.-C. Liberati N.T. Datto M.B. Frederick J.P. Wang X.-F. Mol. Biol. Cell. 1998; 9: 3309-3319Crossref PubMed Scopus (185) Google Scholar). Equal protein amounts of each sample were resolved on a 12% acrylamide-0.5% bisacrylamide sodium dodecyl sulfate gel. Proteins were transferred to Immobilon P transfer membrane (Millipore Corp.). Blocking and antibody incubations were performed in 5% dried milk and 0.1% Tween 20 in phosphate-buffered saline for 1 h at room temperature. Antibodies used were: anti-p21 (Santa Cruz Biotechnology, C-19), p15 (Santa Cruz Biotechnology, C-20), anti-Smad-3 (Santa Cruz Biotechnology, H-2), and horseradish peroxidase-conjugated goat-anti-rabbit (Bio-Rad). Western blots were developed with ECL reagent (Amersham Pharmacia Biotech). Complementary oligonucleotides corresponding to two copies of the Smad3-binding consensus element (GTCTAGAC) were synthesized. Electrophoretic mobility shift assays and nuclear extracts preparation were performed as described previously (8Wong C. Rougier-Chapman E. Frederick J. Datto M. Liberati N. Li J.-M. Wang X.-F. Mol. Cell. Biol. 1999; 19: 1821-1830Crossref PubMed Scopus (230) Google Scholar). The MEK Q56P DNA construct inserted into the retroviral vector pBABE puro was a generous gift from Scott Lowe. Amphotrophic pBabe-puro-MEK Q56P retrovirus was then created using standard techniques (23Counter C.M. Hahn W.C. Wei W. Caddle S.D. Beijersbergen R.L. Lansdorp P.M. Sedivy J.M. Weinberg R.A. Proc. Natl. Acad. Sci. U. S. A. 1998; 95: 14723-14728Crossref PubMed Scopus (564) Google Scholar). A control retrovirus was also generated. HaCaT cells were infected with the retroviruses and grown 10% fetal bovine serum α-minimum essential medium supplemented with 0.6 μg/ml puromycin for (pBabe-puro-MEK Q56P) or 300 μg/ml hygromycin (control) for several days. Cells were plated on coverslips and grown overnight. The next day, cells were treated with TGF-β for 30 min, fixed in paraformaldehyde for 10 min at room temperature, and permeabilized in 0.5% Triton X-100 for 5 min. Slips were then incubated in primary (anti-Smad-3, Santa Cruz Biotechnology, H-2) and secondary antibody for 1 h at 37 °C with frequent washes. Slips were then mounted and photographs taken on a Zeiss confocal microscope. Approximately 200 μg of proteins from cells lysed in ULB were incubated with protein A (Amersham Pharmacia Biotech) and α-Smad3 (Santa Cruz Biotechnology) for immunoprecipitation experiments or glutathione-Sepharose bound GST-p300C (20 μg) for GST pull-down experiments. Binding reactions were for 2 h at 4 °C with continual rotation. The beads were collected and washed three times (3 min/wash) with ULB. Bound proteins were eluted by boiling in 1× Laemmli sample buffer, separated by SDS-PAGE, and subjected to immunoblot analysis. Activation of the MAPK pathway leads to phosphorylation of transcription factor substrates such as Elk-1, c-Jun, and c-Myc and subsequently increases their transactivation potential (reviewed in Refs. 24Hill C.S. Treisman R. Cell. 1995; 80: 199-212Abstract Full Text PDF PubMed Scopus (1198) Google Scholar and 25Davis R.J. Mol. Reprod. Dev. 1995; 42: 459-467Crossref PubMed Scopus (383) Google Scholar). Elk-1 appears to be a good substrate for ERK1/2 in vitro, and its phosphorylation kinetics correlate with MAPK activation. To investigate whether MAPK pathway is activated upon TGF-β treatment, a GAL4-protein fused to Elk-1 and a reporter containing five concatemerized GAL4 sites (5× GAL4) in its promoter were transfected into HaCaT cell. As shown in Fig. 1, TGF-β treatment of transfected cells leads to increased luciferase expression from the 5× GAL4 reporter. This result suggests that TGF-β is potentially able to regulate and activate the MAPK pathway in HaCaT cells. Although overexpression of Smad3 and Smad4 was not sufficient to induce p21 reporter activity, the recent discovery that activation of the Ras pathway could stimulate p21 expression prompted us to test this observation in HaCaT cells, our model system for studying TGF-β signaling. To ascertain whether the MAPK pathway was sufficient to induce p21 reporter activity, we overexpressed a constitutively activated form of MEK (S218/222E) in HaCaT cells and treated the transfected cells with TGF-β. As shown in Fig. 2 A, overexpression of the activated MEK1 not only dramatically increases basal p21 and p15 reporter activity in the absence of TGF-β but also almost completely substitutes for the TGF-β effect, suggesting that TGF-β signaling may overlap with this pathway to stimulate p21 transcription. Because the responsive element in the p21 promoter has been mapped to a GC-rich, Sp1-binding element (21Datto M.B. Yu Y. Wang X.-F. J. Biol. Chem. 1995; 270: 28623-28628Abstract Full Text Full Text PDF PubMed Scopus (399) Google Scholar) and GAL4-Sp1 but not GAL4-Sp3 (22Li J.M. Datto M.B. Shen X. Hu P.P. Yu Y. Wang X.F. Nucleic Acids Res. 1998; 26: 2449-2456Crossref PubMed Scopus (92) Google Scholar) can mediate TGF-β-dependent transcription when using the 5× GAL4 reporter, we tested whether MEK could stimulate GAL4-Sp1 or GAL4-Sp3-mediated transcription. As shown in Fig. 2 B, GAL4-Sp1 but not GAL4-Sp3 mediated transcription activation with expression of the constitutively active MEK (S218/222E). This response parallels the effect of TGF-β. To determine whether overexpression of other members of the Ras-Raf-MEK-MAPK pathway were also able to sufficiently induce p15 and p21 promoter activities, we performed similar experiments with a constitutively membrane-bound form of Raf (Raf-CAAX) and Ras (Ras61L). For both p21 and GAL4-Sp1-dependent transcription, the constitutively active form of Raf activated transcription robustly (Fig. 2). Ras61L also activated transcription from p21 promoter, although the effect was not as strong as that of Raf-CAAX (data not shown). This difference in the ability of Ras and Raf-1 mutants to activate the p21 promoter may be the result of a difference in either the expression levels of those proteins or their intrinsic activity. In contrast, overexpression of MKK4/SEK1 and MKK4 dominant negatives (SEKAL) did not influence basal or TGF-β-dependent p21 reporter activity (Fig. 2). To determine whether overexpression of MEK could increase endogenous expression of p21, we used a retroviral construct containing a MEK point mutant Q56P that is constitutively active (20Lin A.W. Barradas M. Stone J.C. van Aelst L. Serrano M. Lowe S.W. Genes Dev. 1998; 12: 3008-3019Crossref PubMed Scopus (771) Google Scholar). Stable mass populations of infected human fibroblasts with this retrovirus after selection with puromycin express higher levels of p21 (20Lin A.W. Barradas M. Stone J.C. van Aelst L. Serrano M. Lowe S.W. Genes Dev. 1998; 12: 3008-3019Crossref PubMed Scopus (771) Google Scholar). Similar results were obtained in our stable mass populations of HaCaT cells with an increase in endogenous p21 protein levels (Fig. 3 A). Interestingly, although the p21 levels are highly abundant in these cells, they remain viable and appear to divide as rapidly as parental HaCaT cells. However, morphologically, these cells are larger, and there seems to be an increase in extracellular matrix deposition (data not shown). Recent reports suggest that TGF-β and other ligands such as epidermal growth factor and hepatocyte growth factor can induce Smad-2 phosphorylation, translocation, and nuclear function through the MAPKs (26de Caestecker M.P. Parks W.T. Frank C.J. Castagnino P. Bottaro D.P. Roberts A.B. Lechleider R.J. Genes Dev. 1998; 12: 1587-1592Crossref PubMed Scopus (254) Google Scholar). Others suggest that activation of the MAPKs can lead to a Smad phosphorylation event that prevents its nuclear localization (27Kretzschmar M. Doody J. Timokhina I. Massague J. Genes Dev. 1999; 13: 804-816Crossref PubMed Scopus (856) Google Scholar). With our MEK Q56P-infected HaCaT cells, we were able to investigate whether constitutively active MEK could affect Smad signaling and therefore to determine whether the MAPK pathway lay upstream of Smad signaling or could cross-talk and inhibit Smad function. We found that HaCaT cells infected with MEK Q56P behave normally in regard to Smad activity. Upon TGF-β treatment, an inducible complex is still observed in the electrophoretic mobility shift assay with the Smad-binding element as a probe (Fig. 3 B). In addition, the overexpression of MEK Q56P in those cells does not influence the ability of Smads to translocate into the nucleus upon TGF-β treatment (Fig. 3 C). To determine whether TGF-β could exert growth inhibitory effect on those cells, the amount of [3H]thymidine incorporated during a 2-h period was measured after 18 h TGF-β treatment. With Smad signaling not affected by MEK Q56P overexpression, it is not surprising that TGF-β can still potently inhibit the proliferation of those cells (Fig. 3 D). To determine whether the Ras-Raf-MEK-MAPK cascade was required for the ability of TGF-β to induce p21, p15, as well as GAL4-Sp1-mediated transcription, dominant negative Ras (Ras 17A) or Raf (Raf-N4) were co-expressed in HaCaT cells with the p21 minimal promoter reporter (p21P93S), the p15 minimal promoter reporter (p15P113S), or GAL4-Sp1B with the 5× GAL4 minimal reporter and treated with TGF-β for 20–24 h. As shown in Fig. 4 A, the ability of TGF-β to stimulate p21 and p15 promoters was abrogated by co-expression of the dominant negative Ras. Raf-1 dominant negative has a similar effect on the p15 promoter, but to p21 promoter the effect is not as dramatic as that of the Ras dominant negative (Fig. 4 A). Again, this difference in the ability of Ras and Raf-1 dominant negative mutants to block the TGF-β-mediated induction of those promoters may be the result of a difference in either the expression levels of those proteins or their intrinsic activity. Nevertheless, these results suggest that both Ras and Raf are potentially required for TGF-β to stimulate promoter activities of p15 and p21. As controls, the same experiments were performed with a Cdc42 17N dominant negative as well as a Rac 17N dominant negative; in both cases the ability of TGF-β to induce the p21 reporter was not affected (Fig. 4 B). This is similar to a previously reported study where a dominant negative Ras (17N) can block the ability of TGF-β3 to induce p27 and p21 in epithelial cells (28Yue J. Buard A. Mulder K.M. Oncogene. 1998; 17: 47-55Crossref PubMed Scopus (35) Google Scholar). Next, we asked whether MEK was required for TGF-β to stimulate the expression of endogenous p21 and p15. A new MEK inhibitor, U0126 (Promega), was recently developed and found to be more potent than the MEK inhibitor, PD98059, at specifically blocking MEK and ERK activity, without affecting the activity of other ERK family members, p38 and JNK. The availability of this compound allowed us to test the in vivo significance of MEK activity in TGF-β stimulation of p21 and p15 expression. Cells were treated with U0126 at concentrations of 10 or 70 μm for 30 min before treatment with TGF-β for 12 h. As shown in Fig. 4 C, the ability of TGF-β to induce p21 and p15 protein expression was dramatically reduced in the presence of the inhibitor. In contrast, CDK2 levels and basal levels of p21 and p15 remain relatively unchanged. Other specific experimental controls were performed. The ability of Smad3 to bind to Smad4 upon TGF-β treatment (Fig. 4 E) and the ability of Smad3 to bind to GST-p300C (Fig. 4 E) were not affected by incubation with 70 μm U0126, strongly implying that a nonspecific blockage of TGF-β type I receptor kinase activity does not occur at the concentration of 70 μm for the inhibitor U0126. In addition, basal phospho-p38 levels do not change with incubation of 70 μm U0126 (data not shown), suggesting that this inhibitor acts as a specific MEK/MAPK inhibitor. To determine the consequences of the inability of TGF-β to induce p21 and p15 expression, we measured the ability of TGF-β to cause growth inhibition in the presence of the inhibitor using [3H]thymidine labeling. Exponentially growing cells were treated with TGF-β in the presence of increasing concentrations of U0126 for 18 h and pulse-labeled with [3H]thymidine for the final 2 h. As shown in Fig. 4 D, the ability of TGF-β to inhibit growth is reduced with increasing concentrations of U0126. In addition, the cells treated with U0126 do not develop the morphological changes normally associated with TGF-β treatment (data not shown). Our results and those of others suggest that Ras activation may be a part of the TGF-β signaling pathway required for p21 induction. The molecular link between Ras activation and the TGF-β receptor complex, however, remains unknown. Because one mechanism for the activation of Ras by many mitogenic signals often involves the activation of tyrosine kinases, we explored this possibility by using herbimycin A, an inhibitor of tyrosine kinase activity (29Ahn S. Maudsley S. Luttrell L.M. Lefkowitz R.J. Daaka Y. J. Biol. Chem. 1999; 274: 1185-1188Abstract Full Text Full Text PDF PubMed Scopus (244) Google Scholar). As shown in Fig. 5, the ability of TGF-β to increase p21 protein expression is significantly reduced upon incubation of HaCaT cells with 1 μm herbimycin A, suggesting that a tyrosine kinase may be involved in mediating the TGF-β signal. A paradigm is emerging whereby multiple signals use the Ras/Raf/MEK signaling cascade to induce p21 and cause growth arrest. In mammalian cells, signals such as TGF-β, myogenesis, epidermal growth factor, nerve growth factor, high levels of extracellular calcium, a histone deacetylase inhibitor, the mycotoxin fumonsin B1, UV radiation, and the geranylgeranyltransferase I inhibitor GGTI-298 (21Datto M.B. Yu Y. Wang X.-F. J. Biol. Chem. 1995; 270: 28623-28628Abstract Full Text Full Text PDF PubMed Scopus (399) Google Scholar, 30Xiao H. Hasegawa T. Isobe K. J. Cell. Biochem. 1999; 73: 291-302Crossref PubMed Scopus (132) Google Scholar, 31Billon N. Carlisi D. Datto M.B. van Grunsven L.A. Watt A. Wang X.F. Rudkin B.B. Oncogene. 1999; 18: 2872-2882Crossref PubMed Scopus (122) Google Scholar, 32Zhang Y. Dickman M.B. Jones C. J. Biol. Chem. 1999; 274: 12367-12371Abstract Full Text Full Text PDF PubMed Scopus (18) Google Scholar, 33Haapajarvi T. Kivinen L. Heiskanen A. des Bordes C. Datto M.B. Wang X.F. Laiho M. Exp. Cell Res. 1999; 248: 272-279Crossref PubMed Scopus (56) Google Scholar, 34Adnane J. Bizouarn F.A. Qian Y. Hamilton A.D. Sebti S.M. Mol. Cell. Biol. 1998; 18: 6962-6970Crossref PubMed Google Scholar, 35Halevy O. Novitch B.G. Spicer D.B. Skapek S.X. Rhee J. Hannon G.J. Beach D. Lassar A.B. Science. 1995; 267: 1018-1021Crossref PubMed Scopus (1092) Google Scholar, 36Missero C. Calautti E. Eckner R. Chin J. Tsai L.H. Livingston D.M. Dotto G.P. Proc. Natl. Acad. Sci. U. S. A. 1995; 17710: 19244-28491Google Scholar) have all been shown to induce p21 expression as well as growth arrest. In addition, most of these signals appear to signal through Sp1- and Sp3-binding response elements in the p21 promoter. Activation of the MAPK pathway could be a common pathway these signals may utilize to stimulate p21 expression. Interestingly, in budding yeast an analogous situation occurs during pheromone-induced response, where a MAPK-like (STE 20, STE11, STE 7, Fus3/Kss1) pathway is activated, resulting in the phosphorylation of the transcription factor STE12, a subsequent increase in transcription of Far1, the yeast equivalent of a cyclin-dependent kinase inhibitor, and cell cycle arrest (37Herskowitz I. Cell. 1995; 80: 187-197Abstract Full Text PDF PubMed Scopus (867) Google Scholar). Like STE12, phosphorylation of Sp1 by the MAPKs may be one mechanism by which transcription of p21 is increased in these cells. TGF-β can activate different MAPK members to stimulate transcriptional events. In a recent report by Hocevar et al.(38Hocevar B.A. Brown T.L. Howe P.H. EMBO J. 1999; 18: 1345-1356Crossref PubMed Google Scholar), TGF-β was shown to stimulate JNK activity to increase fibronectin synthesis in a Smad4-independent manner. Increases in SAPK/JNK activity have been reported elsewhere (39Atfi A. Djelloul S. Chastre E. Davis R. Gespach C. J. Biol. Chem. 1997; 272: 1429-1432Abstract Full Text Full Text PDF PubMed Scopus (273) Google Scholar), as have increases in ERK (40Frey R.S. Mulder K.M. Cancer Res. 1997; 57: 628-633PubMed Google Scholar, 41Frey R.S. Mulder K.M. Cancer Lett. 1997; 117: 41-50Crossref PubMed Scopus (61) Google Scholar). We have taken advantage of the available phospho-specific antibodies for JNK, p38, and ERK and performed time-dependent Western blots to ascertain which MAPK family member is regulated by TGF-β in our system. Although GAL4-Elk-mediated transcription is indeed increased upon TGF-β treatment, in HaCaT cells these three MAPK family members do not appear to be significantly regulated by TGF-β (data not shown). This result suggests that some other MAPK-like family proteins may be regulated by TGF-β. Although TGF-β-activated kinase, a MAPK kinase kinase homolog responsive to TGF-β, is one such candidate (42Yamaguchi K. Shirakabe K. Shibuya H. Irie K. Oishi I. Ueno N. Taniguchi E. Nishida E. Matsumoto K. Science. 1995; 270: 2008-2011Crossref PubMed Scopus (1178) Google Scholar), expression of a dominant negative of TGF-β-activated kinase does not block the ability of TGF-β to stimulate p21 expression (data not shown). It is intriguing to speculate on how TGF-β turns on the MAPK pathway. Multiple signals can activate MAPK signaling through different pathways. In tyrosine kinase receptor signaling, upon addition of ligand such as platelet-derived growth factor, the receptor protein tyrosine kinase is autophosphorylated and recruits adaptor molecules such as Grb2 and SOS. SOS can, in turn, associate with Ras and which results in Ras becoming GTP-bound. In budding yeast, the serpentine receptors become activated during mating, causing dissociation of Gα from Gβγ, which can then activate a MAPK-like (STE 20, STE11, STE 7, Fus3/Kss1) cascade (37Herskowitz I. Cell. 1995; 80: 187-197Abstract Full Text PDF PubMed Scopus (867) Google Scholar). The ability of tyrosine kinase inhibitor, herbimycin A, to prevent TGF-β induction of p21 suggests that a tyrosine kinase activity is required for its effect. Although specific tyrosine residues on the TGF-β type II receptor may be phosphorylated (Tyr259, Tyr336, and Tyr424) (reviewed in Ref. 43Heldin C.-H. Miyazono K. ten Dijke P. Nature. 1997; 390: 465-471Crossref PubMed Scopus (3358) Google Scholar), it remains to be discovered whether these residues contribute to TGF-β signaling function. Smad proteins play a pivotal role in TGF-β signaling pathway, and here we show that MAPK pathway is also essential for p21 induction by TGF-β. How Smads functionally relate to the MAPK pathway and potentiate growth arrest activity for TGF-β is still unclear. Previous experiments suggest that Smads are required for TGF-β-mediated growth arrest but on the other hand, overexpression of Smads may not be sufficient for the p21 induction. Our data indicate that the MAPK pathway does not function upstream of Smads because cells stably overexpressing constitutively activated MEK Q56P do not show an effect on TGF-β-induced Smad localization or DNA binding. Therefore, the MAPK pathway could be a distinct, parallel pathway from Smad signaling. Alternatively, it could play a role as a downstream effector of Smad signaling, although if the latter scenario is the case, at least another signal input in addition to Smads is probably needed to activate the MAPK/p21 pathway. In certain cells, this other signal may already be constitutively activated, as is likely the case in hepatic cells where overexpressed Smads alone can stimulate p21 promoter activity (44Moustakas A. Kardassis D. Proc. Natl. Acad. Sci. U. S. A. 1998; 95: 6733-6738Crossref PubMed Scopus (321) Google Scholar). Because in HaCaT cells activation of the MAPK pathway alone, but not Smad3/Smad4 overexpression, is sufficient to stimulate p21 expression and in hepatic cells overexpression of Smads alone can also stimulate p21, it is more likely that the MAPK pathway acts downstream of Smads. It is possible that Smad3 can also act as a required intermediate cytoplasmic protein, in addition to its role in the nucleus that mediates the association between the TGF-β receptors and Ras. In supporting this possibility, a recent report showed that Smad3 may associate with other novel cytoplasmic adaptor proteins such as SARA to modulate appropriate TGF-β signaling (45Tsukazaki T. Chiang T.A. Davison A.F. Attisano L. Wrana J.L. Cell. 1998; 95: 779-791Abstract Full Text Full Text PDF PubMed Scopus (794) Google Scholar). We thank R & D Systems for supplying TGF-β1. We are grateful to Dr. J. Horowitz for the GAL4-Sp1B construct, Dr. C. Der for the GAL4-Elk, Ras 17A, Raf-N4, Raf-CAAX, MEKS218/222D, Rac 17N, cdc42 17N, MKK4/SEK-1, and SEK-AL constructs, and Dr. Scott Lowe for the MEK Q56P pBABE-puro construct. In addition, we thank Drs. G. Reuther, R. Plattner, P. Zipfel, K. Courtney, G. Leone, S. Ahn, and A. M. Pendergast for insightful comments and criticisms. Lastly, we thank members of the Wang laboratory for helpful discussions and Yong Yu for excellent technical assistance.
Referência(s)