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In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus

2003; Inter-Research; Volume: 55; Linguagem: Inglês

10.3354/dao055133

1616-1580

Juan Miguel Redondo, Oswaldo Palenzuela, Pilar Álvarez-Pellitero,

Microbial infections and disease research

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 55:133-144 (2003) - doi:10.3354/dao055133 In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus María J. Redondo, Oswaldo Palenzuela*, Pilar Alvarez-Pellitero Consejo Superior de Investigaciones Científicas, Instituto de Acuicultura Torre la Sal, 12595 Ribera de Cabanes, Castellón, Spain *Corresponding author. Email: oswaldo@iats.csic.es ABSTRACT: In vitro cultivation of the myxozoan Enteromyxum scophthalmi was attempted using different culture media and conditions. The progress of the cultures was monitored using dye-exclusion viability counts, tetrazolium-based cell-proliferation assays, measuring the incorporation of BrdU during DNA synthesis, and by morphological studies using light and electron microscopes. In preliminary experiments, the persistence of viable stages for a few days was ascertained in both medium 199 (M199) and in seawater. An apparent initial proliferation was noticed in the culture media, with many young stages observed by Day 7 post-inoculation (p.i.). In contrast, fast degeneration occurred in seawater, with but a few living stages persisting to Day 1 p.i and none to Day 5 p.i. Both tetrazolium-based cell-proliferation assays and dye-exclusion viability counts demonstrated a progressive degeneration of the cultures. Although M199 medium and neutral pH with the addition of sera appeared to provide the most favourable conditions during the first few hours, all cultures degenerated with time and no parasite proliferation or maintenance could be achieved in the long term in any of the conditions assayed, including attempts of co-cultivation with a turbot cell line. The ultrastructure of stages cultured for 15 d demonstrated complete degeneration of organelles and mitochondria, although the plasma membrane remained intact in many stages. Unknown factors related to the metabolism or the life cycle of this myxozoan are probably responsible for the inability to culture the parasite, which seems to be strictly dependent on the target host tissues for survival. KEY WORDS: Myxozoa · In vitro cultivation · Parasites · Life cycle Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 55, No. 2. Online publication date: July 08, 2003 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2003 Inter-Research.