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An inactivated vaccine for the control of bluetongue virus serotype 16 infection in sheep in Italy

2007; Elsevier BV; Volume: 124; Issue: 1-2 Linguagem: Inglês

10.1016/j.vetmic.2007.04.017

1873-2542

Giovanni Savini, Gaetano Federico Ronchi, Alessandra Leone, Antonella Ciarelli, P Migliaccio, Paola Franchi, Maria Teresa Mercante, A. Pini,

Viral Infections and Vectors

Because no suitable products are at the moment available to safely control the spread of BTV-16 in Europe, an inactivated vaccine was produced from the reference field isolate of bluetongue virus serotype 16. One group of six sheep was vaccinated subcutaneously with the inactivated vaccine twice, on days 0 and 28, whereas a second group of eight sheep was inoculated with saline solution and used as mock-vaccinated control animals. Seventy-eight days after the first vaccination, all sheep were inoculated subcutaneously with a suspension containing 106.3 TCID50 of a virulent reference BTV-16 isolate. Apart from a transient inflammatory reaction at the injection site, no adverse effects were reported following vaccination. All vaccinated animals developed high titres (7.3–9.3 log2(ED50%/50 μl)) of virus-specific neutralising antibodies and were resistant to challenge with BTV-16. Conversely, following challenge, control animals developed hyperthermia and long lasting high-titre viraemia.