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BIBTEX RIS

Using multiplex real time PCR in order to streamline a routine diagnostic service

2008; Elsevier BV; Volume: 43; Issue: 4 Linguagem: Inglês

10.1016/j.jcv.2008.08.020

ISSN

1873-5967

Autores

Rory Gunson, S. Bennett, Andrew G. MacLean, William F. Carman,

Tópico(s)

Animal Disease Management and Epidemiology

Resumo

An increasing number of virology laboratories are now utilising in house real time PCR assays as the frontline diagnostic tests. As the number of tests on offer increases the natural progression from this will be to rationalise their service via multiplexing. Since 2003 we have introduced a large number of qualitative and quantitative multiplex real time PCR assays into our routine testing service. This paper describes the development of the multiplex assays, the problems encountered and the resultant benefits to the routine service.

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