In vitro activation of human sperm induced by amphibian egg extract
1985; Elsevier BV; Volume: 157; Issue: 2 Linguagem: Inglês
10.1016/0014-4827(85)90126-0
ISSN1090-2422
AutoresKatherine Gordon, David B. Brown, Frank H. Ruddle,
Tópico(s)Reproductive biology and impacts on aquatic species
ResumoIn this paper we characterize a system for the activation of human sperm using cell-free extracts from Xenopus laevis eggs. We characterize the kinetics of sperm activation in terms of morphological and DNA synthetic parameters. We have optimized some of the components which are necessary for chromatin decondensation and present data to demonstrate that the in vitro process is efficient over a wide range of salt concentrations and amounts of supplementary reducing agents.
Referência(s)