Eupatilin inhibits lipopolysaccharide-induced expression of inflammatory mediators in macrophages
2011; Elsevier BV; Volume: 88; Issue: 25-26 Linguagem: Inglês
10.1016/j.lfs.2011.04.011
ISSN1879-0631
AutoresEun‐Ju Choi, Soyoung Lee, Jeong-Ryong Chae, Hyun‐Shik Lee, Chang‐Duk Jun, Sang‐Hyun Kim,
Tópico(s)Natural product bioactivities and synthesis
ResumoEupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone) is a pharmacologically active ingredients in Stillen(TM), a drug for the gastric mucosal ulcers. Eupatilin has been known to possess anti-peptic, anti-cancer, and anti-allergy activity. In this report, we defined the effect of eupatilin on the endotoxin-induced inflammation in lipopolysaccharide (LPS)-stimulated macrophages.Mouse J774A.1 cell line and mouse peritoneal macrophages were used. Gene expression and production of inflammatory mediators were determined by real-time PCR and Western blot.Eupatilin dose-dependently suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO). Eupatilin decreased LPS-induced expression of inflammatory mediators and pro-inflammatory cytokines such as cyclooxygenase-2, monocyte chemoattractant protein-1, tumor necrosis factor-α, interleukin (IL)-1β and IL-6. In addition, this suppression of inflammatory mediators was nuclear factor (NF)-κB dependent.Our findings imply that eupatilin suppresses inflammatory responses by the inhibition of NF-κB signaling pathway, and downstream inflammatory mediators in endotoxin-stimulated macrophages.
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