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Systematic study of ternary solvent behaviour in reversed-phase liquid chromatography

1981; Elsevier BV; Volume: 218; Linguagem: Inglês

10.1016/s0021-9673(00)82060-9

1873-3778

Peter J. Schoenmakers, H.A.H. Billiet, L. de Galan,

Crystallization and Solubility Studies

An extensive experimental survey of the retention behaviour of 32 solutes in the system methanol-tetrahydrofuran-water and 49 solutes in the system methanol-acetonitrile-water is presented. The retention data are fitted to a second order six-parameter equation, describing the capacity factor as a function of mobile phase composition. Iso-eluotropic lines, i.e., lines that connect solvents of equal eluotropic strength, are constructed in the ternary diagrams for the two systems and compared with theoretical lines, predicted from solubility parameter theory. Specific effects are defined as variations in retention for a particular solute, using solvents of equal eluotropic strength. Such effects appear to be larger between different binary mixtures than within the ternary triangle. Ternary solvents thus provide a smooth transition between two limiting binary mixtures.