Portal de Periódicos da CAPES

Induction of immunoglobulin-producing human peripheral blood lymphocytes in serum-free medium

1982; Elsevier BV; Volume: 52; Issue: 2 Linguagem: Inglês

10.1016/0022-1759(82)90052-7

1872-7905

Yasuo Tanno, Sumio Arai, Tamotsu Takishima,

Protein purification and stability

Induction of immunoglobulin-secreting cells from human peripheral blood lymphocytes in a serum-free culture medium was studied. Albumin, transferrin, insulin and fibronectin can replace serum entirely for support of pokeweed mitogen (PWM)-stimulated B lymphocytes, measured by a reverse hemolytic plaque assay using protein A-coated red cells. In this serum-free system, growth and maturation to IgM and IgG secretion occur at the same or higher efficiency as in conventional serum-containing medium, with maximum numbers of plaque-forming cells on day 6 at optimal dose of PWM, 0.5-5 micrograms/ml. This system can be used to avoid the interference from undefined serum components.