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Long‐Term Stabilization of the Activity of Ascorbate Oxidase Adsorbed on a Porous Carbon Material by Polymaleimidostyrene

2006; Taylor & Francis; Volume: 40; Issue: 3 Linguagem: Inglês

10.1080/00032710600964734

1532-236X

Ryuta TOMITA, Kentaro KOKUBUN, Teruki Hagiwara, S. Uchiyama,

Advanced Nanomaterials in Catalysis

Abstract The biological catalysts adsorbed on the carbon surface gradually lost their activities. In order to prolong stabilization of the enzyme activity, polymaleimidostyrene (PMS) was used to preserve enzyme conformation. Sulfhydryl and amino groups of enzyme or biological tissue can be covalently bound to PMS. By using the carbon material coated by PMS, the stability of L‐ascorbic acid sensor response using purified enzyme was significantly improved, and its initial response was not decreased at all for 150 days, although the purified enzymes adsorbed to the carbon without PMS lost their activity 22.5% after 10 days.