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Indexing and production of virus-free chrysanthemums

2005; Institute of Experimental Botany of the Czech Academy of Sciences; Volume: 49; Issue: 1 Linguagem: Inglês

10.1007/s10535-005-0152-0

1573-8264

Raja Ram, Neeraj Verma, Amit Kumar Singh, Lokesh Singh, Vipin Hallan, A. A. Zaidi,

Plant Parasitism and Resistance

Chrysanthemum morifolium cv. Regol Time plants were found to be infected with Chrysanthemum B carlavirus (CVB). They were made CVB-free by using meristem tip culture, chemotherapy and thermotherapy. The plants were indexed by biological assay, double antibody sandwitch enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The maximum number of virus-free plants (26.7 %, as indexed by RT-PCR) was obtained with 2-thiouracil at 0.04 g dm-3 concentration. Only 12 % plants were found to be virus-free, after being kept at 38 °C for 30 d. For indexing CVB in chrysanthemums, RT-PCR was found to be the most reliable method.