pH opposite effects on synthesis of dinucleoside polyphosphates and on oxidation reactions catalyzed by firefly luciferase
2003; Wiley; Volume: 543; Issue: 1-3 Linguagem: Inglês
10.1016/s0014-5793(03)00382-x
ISSN1873-3468
AutoresHugo Fraga, Joaquim C. G. Esteves da Silva, Rui Fontes,
Tópico(s)Photoreceptor and optogenetics research
ResumoPrevious results have shown that an oxidizing product of firefly luciferin, dehydroluciferyl‐adenylate, is the main intermediate in the process of synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase (EC 1.13.12.7). However, we have found that the pH effects on the luciferase oxidizing processes and on the synthesis of dinucleoside polyphosphate are opposite: acidic assay media enhance the synthesis of dinucleoside polyphosphate and inhibit the oxidizing processes. The reason for this apparent contradiction lies on the activation effect of low pH on the adenylate transfer reaction from dehydroluciferyl‐adenylate to the acceptor nucleotide.
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