Ornithogalum: a new host of Cucumber mosaic virus (CMV) from India
2005; Wiley; Volume: 54; Issue: 2 Linguagem: Inglês
10.1111/j.1365-3059.2005.01128.x
ISSN1365-3059
AutoresNeeraj Verma, Lokesh Singh, Amit Kumar Singh, Saurabh Kulshrestha, G. Raikhy, Vipin Hallan, Raja Ram, A. A. Zaidi,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoPlant PathologyVolume 54, Issue 2 p. 256-256 Free Access Ornithogalum: a new host of Cucumber mosaic virus (CMV) from India N. Verma, N. Verma Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorL. Singh, L. Singh Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorA. K. Singh, A. K. Singh Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorS. Kulshrestha, S. Kulshrestha Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorG. Raikhy, G. Raikhy Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorV. Hallan, V. Hallan Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorR. Ram, R. Ram Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorA. A. Zaidi, A. A. Zaidi Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, India E-mail: zaidi_aijaz@yahoo.comSearch for more papers by this author N. Verma, N. Verma Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorL. Singh, L. Singh Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorA. K. Singh, A. K. Singh Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorS. Kulshrestha, S. Kulshrestha Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorG. Raikhy, G. Raikhy Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorV. Hallan, V. Hallan Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorR. Ram, R. Ram Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, IndiaSearch for more papers by this authorA. A. Zaidi, A. A. Zaidi Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur−176 061, Himachal Pradesh, India E-mail: zaidi_aijaz@yahoo.comSearch for more papers by this author First published: 21 March 2005 https://doi.org/10.1111/j.1365-3059.2005.01128.xCitations: 12 AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Ornithogalum thyrsoides (family Hyacinthaceae) is an ornamental cut flower crop which has a long vase life and is resistant to extreme temperatures. Plants have been reported to be infected with Ornithogalum mosaic virus (OMV) and Tobacco necrosis virus (TNV) (Burger & Wechmar, 1995). Ornithogalum plants growing in a floriculture field at the Institute of Himalayan Bioresource Technology, Palampur, were found exhibiting severe mosaic on the leaves. The causal virus was found to be transmitted mechanically, causing local lesions on Chenopodium album and C. amaranticolor, and systemic mosaic on Nicotiana clevelandii, N. glutinosa, N. megalosiphon and N. tabacum cv. White Burley. In ELISA, the virus reacted with Cucumber mosaic virus (CMV)-specific antibodies procured from Agdia (USA). Leaf dip preparations from virus-infected plants in electron microscopy revealed the presence of ∼29 nm isometric particles. In immunoelectron microscopy (IEM), the virus was readily trapped and decorated with CMV-specific antibodies. Total RNA was isolated from healthy and naturally infected ornithogalum, as well as from inoculated plants, using RNAqueous (Ambion, USA) as per the manufacturer's instructions. Reverse transcription-polymerase chain reaction (RT-PCR) was performed using the CPTALL primer pair (Choi et al., 1999). Amplicons of expected size (∼950 bp) were obtained in virus-infected and inoculated plants. No amplification was observed in healthy ornithogalum plants. PCR-amplified products were cloned and sequenced (AJ745092). BLAST searches revealed the nucleotide and the deduced amino acid sequence showing 97 and 93–95% similarity with the available sequences of CMV subgroup II, respectively, confirming the association of CMV with the severe mosaic on ornithogalum. This is the first definitive report of CMV infecting ornithogalum. References Burger JT, Wechmar MBV, 1995. Ornithogalum. In: G Loebenstein, RH Lawson, AA Brunt, eds. Virus and Virus-like Diseases of Bulb and Flower Crops. Chichester, UK: John Wiley, 341– 5. Google Scholar Choi SK, Choi JK, Park WM, Ryu KH, 1999. RT-PCR detection and identification of three species of cucumovirus with a genus-specific single pair of primers. Journal of Virological Methods 83, 67– 73. CrossrefCASPubMedWeb of Science®Google Scholar Citing Literature Volume54, Issue2April 2005Pages 256-256 ReferencesRelatedInformation
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