Analysis of amino acids by capillary zone electrophoresis with electrochemical detection
2002; Wiley; Volume: 23; Issue: 4 Linguagem: Inglês
10.1002/1522-2683(200202)23
ISSN1522-2683
AutoresQian Dong, Wenrui Jin, Jihao Shan,
Tópico(s)Analytical Chemistry and Chromatography
ResumoELECTROPHORESISVolume 23, Issue 4 p. 559-564 Research Article Analysis of amino acids by capillary zone electrophoresis with electrochemical detection Qian Dong, Qian Dong School of Chemistry and Chemical Engineering and State Key Laboratory of Microbial Technology, Shandong University, Jinan, ChinaSearch for more papers by this authorWenrui Jin, Corresponding Author Wenrui Jin [email protected] School of Chemistry and Chemical Engineering and State Key Laboratory of Microbial Technology, Shandong University, Jinan, ChinaSchool of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China Fax: +86-531-8565167===Search for more papers by this authorJihao Shan, Jihao Shan School of Chemistry and Chemical Engineering and State Key Laboratory of Microbial Technology, Shandong University, Jinan, ChinaSearch for more papers by this author Qian Dong, Qian Dong School of Chemistry and Chemical Engineering and State Key Laboratory of Microbial Technology, Shandong University, Jinan, ChinaSearch for more papers by this authorWenrui Jin, Corresponding Author Wenrui Jin [email protected] School of Chemistry and Chemical Engineering and State Key Laboratory of Microbial Technology, Shandong University, Jinan, ChinaSchool of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China Fax: +86-531-8565167===Search for more papers by this authorJihao Shan, Jihao Shan School of Chemistry and Chemical Engineering and State Key Laboratory of Microbial Technology, Shandong University, Jinan, ChinaSearch for more papers by this author First published: 20 February 2002 https://doi.org/10.1002/1522-2683(200202)23:4 3.0.CO;2-GCitations: 36AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The precapillary derivatization of 20 amino acids with naphthalene-2,3-dicarboxaldehyde (NDA) and CN– was investigated. All these derivatized amino acids could be oxidized on the carbon fiber microdisk bundle electrode except proline. Capillary zone electrophoresis with electrochemical detection was employed for the analysis of 19 amino acids. The optimum conditions of separation and detection were borate, pH 9.48, for the electrolyte, 18 kV for the separation voltage and 1.15 V versus a saturated calomel electrode for the detection potential. Limits of detection of concentration or mass for individual amino acids were between 1.7×10–7 and 1.8×10–6 mol/L or 84 and 893 amol (according to the signal-to-noise ratio of 3) for the injection voltage of 6 kV and injection time of 10 s. The relative standard deviations were between 0.80 and 2.3% for the migration times and 1.4 and 6.4% for the electrophoretic peak currents. From a mixture of 19 amino acids, 10 amino acids (Arg, Lys, Orn, Try, Ser, Ala, Gly, Cys, Glu, Asp) could be well separated. The other 9 amino acids appeared on three electrophoretic peaks. From the samples, in which the nine amino acids do not exist simultaneously, some of them could also be detected. The method was applied to the determination of amino acids in beer by the standard addition method. The recovery for the amino acids in beer was 91–109%. REFERENCES 1 Roach, M. C., Harmony, M. D., Anal. Chem. 1987, 59, 411–415. 10.1021/ac00130a007 CASPubMedWeb of Science®Google Scholar 2 Oates, M. D., Cooper, B. R., Jorgenson, J. W., Anal. Chem. 1990, 62, 1573–1577. 10.1021/ac00214a006 CASPubMedWeb of Science®Google Scholar 3 Novotny, M., J. Microcol. Sep. 1990, 2, 7–20. 10.1002/mcs.1220020105 CASGoogle Scholar 4 Novotny, M. V., Cobb, K. 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