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A fast screening method for the identification of siderophores from fluorescentPseudomonas spp. by liquid chromatography/electrospray mass spectrometry

1999; Wiley; Volume: 34; Issue: 4 Linguagem: Inglês

10.1002/(sici)1096-9888(199904)34

1096-9888

S. Kilz, Ch. Lenz, Regine Fuchs, H. Budzikiewicz,

Bacterial biofilms and quorum sensing

Journal of Mass SpectrometryVolume 34, Issue 4 p. 281-290 Research Article A fast screening method for the identification of siderophores from fluorescent Pseudomonas spp. by liquid chromatography/electrospray mass spectrometry S. Kilz, S. Kilz Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, GermanySearch for more papers by this authorCh. Lenz, Ch. Lenz Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, GermanySearch for more papers by this authorR. Fuchs, R. Fuchs Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, GermanySearch for more papers by this authorH. Budzikiewicz, Corresponding Author H. Budzikiewicz [email protected] Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, GermanyInstitut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, Germany===Search for more papers by this author S. Kilz, S. Kilz Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, GermanySearch for more papers by this authorCh. Lenz, Ch. Lenz Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, GermanySearch for more papers by this authorR. Fuchs, R. Fuchs Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, GermanySearch for more papers by this authorH. Budzikiewicz, Corresponding Author H. Budzikiewicz [email protected] Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, GermanyInstitut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Cologne, Germany===Search for more papers by this author First published: 19 May 1999 https://doi.org/10.1002/(SICI)1096-9888(199904)34:4 3.0.CO;2-MCitations: 39 Part LXXIX of the series ‘‘Bacterial Constituents;’’ for part LXXVIII see Ref. 16. This paper is dedicated to the memory of Prof. Dr Wilhelm Richter AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract A screening method was developed for the fast identification of known pyoverdin-type siderophores produced by fluorescent Pseudomonas spp. It is based on reversed-phase high-performance liquid chromatography interfaced with electrospray ionization mass spectrometry of the Sep-Pak RP-C18 culture supernatant extracts. The siderophores of five bacterial strains were characterized by their molecular masses obtained from their doubly protonated molecular ions [M+2H]2+ and their UV/visible spectra recorded with a diode-array detector. Additional structural information was gained by skimmer collision-induced dissociation experiments. For all strains new minor siderophores were found. A table of fully or partially identified pyoverdins and related siderophores is provided which will be the basis for screening studies. Copyright © 1999 by John Wiley & Sons, Ltd. REFERENCES 1. H. Budzikiewicz, Z. Naturforsch., Teil C 52, 713 (1997). 2. G. Briskot, K. Taraz and H. Budzikiewicz, Liebigs Ann. Chem. 375 (1989). 3. H. Budzikiewicz, in Studies in Natural Product Chemistry, edited by , Vol. 19, p. 793. Elsevier, Amsterdam (1997). 4. J.-M. Meyer, A. Stintzi, D. De Vos, P. Cornelis, R. Tappe, K. Taraz and H. Budzikiewicz, Microbiology 143, 35 (1997). 5. K. Botzenhart and H. Rüden, Antibiot. 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