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Molecular characterisation and relative incidence of bean- and soybean-infecting begomoviruses in northwestern Argentina
2010; Wiley; Volume: 158; Issue: 1 Linguagem: Inglês
10.1111/j.1744-7348.2010.00441.x
ISSN1744-7348
AutoresPatricia Elsa Rodríguez-Pardina, Kentaro Hanada, I. G. Laguna, F. Murilo Zerbini, Daniel A. Ducasse,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoAnnals of Applied BiologyVolume 158, Issue 1 p. 69-78 Molecular characterisation and relative incidence of bean- and soybean-infecting begomoviruses in northwestern Argentina P.E. Rodríguez-Pardina, Corresponding Author P.E. Rodríguez-Pardina INTA – Instituto de Fitopatologia y Fisiologia Vegetal, Camino 60 Cuadras Km 5 1/2 (5119) Córdoba, ArgentinaP.E. Rodriguez-Pardina, INTA – Instituto de Fitopatologia y Fisiologia Vegetal, Camino 60 Cuadras Km 5 1/2 (5119) Córdoba, Argentina.Email: prodriguezp@correo.inta.gov.arSearch for more papers by this authorK. Hanada, K. Hanada National Agricultural Research Center, Kannondai 3-1-1,Tsukuba 305-8666, JapanSearch for more papers by this authorI.G. Laguna, I.G. Laguna INTA – Instituto de Fitopatologia y Fisiologia Vegetal, Camino 60 Cuadras Km 5 1/2 (5119) Córdoba, ArgentinaSearch for more papers by this authorF.M. Zerbini, F.M. Zerbini Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG 36570-000, BrazilSearch for more papers by this authorD.A. Ducasse, D.A. Ducasse INTA – Instituto de Fitopatologia y Fisiologia Vegetal, Camino 60 Cuadras Km 5 1/2 (5119) Córdoba, ArgentinaSearch for more papers by this author P.E. Rodríguez-Pardina, Corresponding Author P.E. Rodríguez-Pardina INTA – Instituto de Fitopatologia y Fisiologia Vegetal, Camino 60 Cuadras Km 5 1/2 (5119) Córdoba, ArgentinaP.E. Rodriguez-Pardina, INTA – Instituto de Fitopatologia y Fisiologia Vegetal, Camino 60 Cuadras Km 5 1/2 (5119) Córdoba, Argentina.Email: prodriguezp@correo.inta.gov.arSearch for more papers by this authorK. Hanada, K. Hanada National Agricultural Research Center, Kannondai 3-1-1,Tsukuba 305-8666, JapanSearch for more papers by this authorI.G. Laguna, I.G. Laguna INTA – Instituto de Fitopatologia y Fisiologia Vegetal, Camino 60 Cuadras Km 5 1/2 (5119) Córdoba, ArgentinaSearch for more papers by this authorF.M. Zerbini, F.M. Zerbini Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG 36570-000, BrazilSearch for more papers by this authorD.A. Ducasse, D.A. Ducasse INTA – Instituto de Fitopatologia y Fisiologia Vegetal, Camino 60 Cuadras Km 5 1/2 (5119) Córdoba, ArgentinaSearch for more papers by this author First published: 08 November 2010 https://doi.org/10.1111/j.1744-7348.2010.00441.xCitations: 26Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Recent studies identified three begomoviruses infecting soybean and bean crops in northwestern (NW) Argentina, bean golden mosaic virus (BGMV), a virus with high capsid protein identity with Sida mottle virus (SiMoV) and a possible new viral species (isolate A). Analysis of complete DNA-A sequences confirmed that isolate A represents a new viral species for which the authors propose the name soybean blistering mosaic virus (SbBMV), whereas the SiMoV-like virus is actually an isolate of tomato yellow spot virus (ToYSV). Molecular hybridisation-based detection of the three begomoviruses was accomplished using a general probe obtained by mixing full-length DNA-A clones of the three begomoviruses and specific probes comprising part of the common region of each viral genome. These probes were used to test samples collected in NW Argentina from 2004 through 2007. Fifty-three percent of the bean samples were infected with BGMV, 11.5% with ToYSV and 9% with SbBMV. For soybean samples, 33% were infected with SbBMV and 18% with ToYSV. BGMV was not detected in soybean. ToYSV was also detected in the wild species Abutilon theophrasti. Citing Literature Supporting Information Supporting Information Additional Supporting Information may be found in the online version of this article: Figure S1 (a) Soybean sample infected with SbBMV. (b) Bean sample infected with ToYSV. (c) Bean sample coinfected with ToYSV and SbBMV. Infections were confirmed by dot-blot hybridization with virus-specific probes under high stringency conditions. Filename Description AAB_441_sm_f1.pdf180.1 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume158, Issue1January 2011Pages 69-78 RelatedInformation
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