Produção Nacional
Lipophorin from Rhodnius prolixus: Purification and partial characterization
1989; Elsevier BV; Volume: 19; Issue: 2 Linguagem: Inglês
10.1016/0020-1790(89)90086-3
ISSN1879-2928
AutoresKátia C. Gondim, Pedro L. Oliveira, Heloisa S.L. Coelho, Hatisaburo Masuda,
Tópico(s)Physiological and biochemical adaptations
ResumoThe lipophorin of adult females of Rhodnius prolixus was radioactively labelled with 32P exclusively in the phospholipid moiety and purified on a KBr ultracentrifugation gradient. The density of purified [32P]phospholipid labelled lipophorin on the fifth day after a blood meal was 1.1211 ± 0.0017 g/ml. By weight it contained 51.7% protein, 0.7% sugar and 47.6% lipid. The protein moiety was composed of three apoproteins of 226 ± 11, 86 ± 2 and 16 ± 1 kDa. Mannose and N-acetylglucosamine were the only sugars detected. Among the lipids, 66.3% were neutral lipids and 33.8% were phospholipids. Analysis by thin-layer chromatography showed that in the total phospholipids fraction 32P was distributed as follows: phosphatidylethanolamine (54.4%), phosphatidylcholine (44.7%), cardiolipin (2.1%), phosphatidylserine (0.7%), phosphatidylinositol (0.4%), sphingomyelin (0.3%) and phosphatidic acid (0.2%). The total phosphate content was 0.53 ± 0.03 nmol/μg of protein.
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