Inorganic pyrophosphate: Fructose-6-phosphate 1-phosphotransferase of the potato tuber is related to the major ATP-dependent phosphofructokinase of E. coli
1988; Elsevier BV; Volume: 154; Issue: 1 Linguagem: Inglês
10.1016/0006-291x(88)90657-2
ISSN1090-2104
AutoresXiaohua Yuan, Danuta Kwiatkowska, Robert G. Kemp,
Tópico(s)Polyamine Metabolism and Applications
ResumoA procedure was developed for the purification of inorganic pyrophosphate: fructose-6-phosphate 1-phospho-transferase (PPi-PFK) from potato tubers. The enzyme has the structure α4β4 with a subunit of 68 kDa and a β subunit of 60 kDa. The structural relationship of this enzyme to other PFKs and to fructose bisphosphatase was examined by immunoprecipitation and immunoblotting. Antibodies to the plant enzyme did not react with E.coli PFK. No cross-reaction was seen among the following enzymes or their antibodies: yeast fructose bisphosphatase; rabbit PFKs A,B, or the enzyme from brain; and the two subunits of the potato PPi-PFK. On the other hand, antibody to E.coli PFK-1 strongly cross-reacts with the 60 kDa polypeptide but not 68 kDa peptide.
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