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Structure of the oligosaccharides from mammalian glycolipids

1971; Elsevier BV; Volume: 7; Issue: 1-2 Linguagem: Inglês

10.1016/0009-3084(71)90019-3

1873-2941

Jun’ichi Kawanami, Takashi Tsuji,

Glycosylation and Glycoproteins Research

Ceramide trihexosides and globosides were isolated from mouse Nakahara-Fukuoka sarcoma, porcine spleen, and rat kidney. The oligosaccharide portions were liberated from the glycolipids by ozonolysis and successive alkali fragmentation by Wiegandt's method. The oligosaccharides were purified by charcoal chromatography, followed by reduction with sodium borohydride. Reduced oligosaccharides were subjected to 220 MHz p.m.r. spectroscopy. The spectra of the oligosaccharides showed clearly resolved doublets for the anomeric protons, confirming the previous finding that the anomeric configuration of the terminal galactose in the ceramide trihexoside is indeed an α-configuration. Further, the structure of the globosides was studied by the above technique and with specific N-acetyl-β-hexosaminidase. From the results, the structure of rat renal and porcine splenic (cytolipin K) globosides were shown to be N-acetylgalactosaminyl (β1→3) galactosyl (α1→4) galactosyl (β1→4) glucosyl ceramide.