Biosynthesis of gibberellins A12, A15, A24, A36, and A37 by a cell-free system from Cucurbita maxima
1974; Elsevier BV; Volume: 13; Issue: 8 Linguagem: Inglês
10.1016/0031-9422(74)80304-3
ISSN1873-3700
AutoresJan E. Graebe, Peter Hedden, Paul Gaskin, Jake MacMillan,
Tópico(s)Advances in Cucurbitaceae Research
ResumoGA12-aldehyde obtained from mevalonate via ent-kaurene, ent-kaurenol, ent-kaurenoic acid and ent-7α-hydroxykaurenoic acid in a cell-free system from immature seeds of Cucurbita maxima was converted to GA12 by the same system. When Mn2+ was omitted from the system GA12-aldehyde and GA12 were converted further to several products. Among these GA15, GA24, GA36 and GA37 were conclusively identified by GC-MS. With the exception of GA37 these GAs have not previously been found in higher plants. Another biosynthetic pathway led from ent-7α-hydroxykaurenoic acid to very polar products via what was tentatively identified as ent-6α, 7α-dihydroxykaurenoic acid. An unidentified component with an MS resembling that of a dihydroxykaurenolide was also obtained from incubations with mevalonate.
Referência(s)