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Homology of IysS and IysU , the two Escherichia coli genes encoding distinct Iysyl-tRNA synthetase species

1990; Oxford University Press; Volume: 18; Issue: 2 Linguagem: Inglês

10.1093/nar/18.2.305

1362-4962

Françoise Lévêque, Pierre Plateau, Phillippe Dessen, Sylvain Blanquet,

Genomics and Phylogenetic Studies

In Escherichia coli , two distinct lysyl-tRNA synthetase species are encoded by two genes: the constitutive lysS gene and the thermoinducible lysU gene. These two genes have been isolated and sequenced. Their nucleotide and deduced amino acid sequences show 79% and 88% identity, respectively. Codon usage analysis indicates the lysS product being more efficiently translated than the lysU one. In addition, the lysS sequence exactly coincides with the sequence of lysS , a gene which is part of the prfB - herC operon. In contrast to the recent proposal of Gampel and Tzagoloff (1989, Proc. Natl. Acad. Sci. USA 86, 6023-6027), the lysU sequence is distinct from the open reading frame located adjacent to frdA , although large homologies are shared by these two genes.