The relationship between methylenetetrahydrofolate reductase c.677TT genotype and oligozoospermia in infertile male patients living in the Trakya region of Turkey
2014; Wiley; Volume: 47; Issue: 9 Linguagem: Inglês
10.1111/and.12380
ISSN1439-0272
AutoresHakan Gürkan, Hilmi Tozkır, Ebru Göncü, Selma Demır, Metin Yazar,
Tópico(s)Sperm and Testicular Function
ResumoAndrologiaVolume 47, Issue 9 p. 1068-1074 Original Article The relationship between methylenetetrahydrofolate reductase c.677TT genotype and oligozoospermia in infertile male patients living in the Trakya region of Turkey H. Gurkan, Corresponding Author H. Gurkan Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, Turkey Correspondence Dr Hakan Gurkan, Department of Medical Genetics, Medical Faculty, Trakya University, 22030 Edirne, Turkey. Tel.: +90-284-2357642-2330; Fax: +90-284-235-3451; E-mail: [email protected]Search for more papers by this authorH. Tozkır, H. Tozkır Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, TurkeySearch for more papers by this authorE. Göncü, E. Göncü Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, TurkeySearch for more papers by this authorS. Ulusal, S. Ulusal Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, TurkeySearch for more papers by this authorM. Yazar, M. Yazar Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, TurkeySearch for more papers by this author H. Gurkan, Corresponding Author H. Gurkan Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, Turkey Correspondence Dr Hakan Gurkan, Department of Medical Genetics, Medical Faculty, Trakya University, 22030 Edirne, Turkey. Tel.: +90-284-2357642-2330; Fax: +90-284-235-3451; E-mail: [email protected]Search for more papers by this authorH. Tozkır, H. Tozkır Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, TurkeySearch for more papers by this authorE. Göncü, E. Göncü Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, TurkeySearch for more papers by this authorS. Ulusal, S. Ulusal Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, TurkeySearch for more papers by this authorM. Yazar, M. Yazar Department of Medical Genetics, Medical Faculty, Trakya University, Edirne, TurkeySearch for more papers by this author First published: 26 November 2014 https://doi.org/10.1111/and.12380Citations: 13Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary Methylenetetrahydrofolate reductase (MTHFR), the key enzyme of the folate metabolic pathway, has been reported to be five times more active in the testicles compared to other organs in adult mice. The aim of this study was to investigate the relationship between MTHFR c.677C>T and c.1298A>C polymorphisms and infertility in nonobstructive azoospermic and oligozoospermic male patients living in the Trakya region of Turkey. The study population included 75 nonobstructive azoospermic and 62 oligozoospermic, nonconsanguineous patients who were referred to the Department of Medical Genetics of Trakya University between 01.03.2012 and 01.06.2013 due to infertility and who had been diagnosed based on clinical examinations and spermiograms. All of the patients had a normal karyotype without a Y chromosome microdeletion. Melting curve analysis with labelled probes and primers that were designed by the manufacturers and the real-time polymerase chain reaction method were used. The MTHFR c.677TT genotype frequency in the oligozoospermic infertile male patient group was greater than that of the fertile control group [odds ratio (OR) = 2.675 (95% CI: 0.979–7.305), (P < 0.048)]. The MTHFR c.677TT genotype may be a genetic risk factor for oligozoospermic infertile male patients who live in the Trakya region of Turkey. References Bezold G, Lange M, Peter RU (2001) Homozygous methylenetetrahydrofolate reductase C677T mutation and male infertility. N Engl J Med 344: 1172– 1173. Chellat D, Rezgoune ML, Hamane D, Semmame O, Benlatrèche C, Abadi N, Satta D (2012) Influence of methylenetetrahydrofolate reductase C677T gene polymorphisms in Algerian infertile men with azoospermia or severe oligozoospermia. Genet Test Mol Biomarkers 16: 874– 878. 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