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Effect of PA-MSHA vaccine on plasma phospholipids metabolic profiling and the ratio of Th2/Th1 cells within immune organ of mouse IgA nephropathy

2006; Elsevier BV; Volume: 43; Issue: 2 Linguagem: Inglês

10.1016/j.jpba.2006.07.040

1873-264X

Lewen Jia, Chang Wang, Hongwei Kong, Jun Yang, Fanglou Li, Shen Lv, Guowang Xu,

Renal Diseases and Glomerulopathies

Phospholipids as a class of important constituents in the biomembranes have been paid increasing attention in many fields. IgA nephropathy is now generally known to be the most common form of primary glomerulonephritis in the world. However, phospholipids metabolism in IgA nephropathy was not clear. Until recently, there was no effective treatment available for patients with IgA nephropathy. In this paper, effect of PA-MSHA vaccine on plasma phospholipid metabolic profile of mouse IgA nephropathy was investigated using high performance liquid chromatography/mass spectrometry (HPLC/MS) and principal components analysis (PCA). Female Balb/c mice were divided into four groups: model group, control group, PA-MSHA treatment group and medicine control group (dipyridamole + common threewingnut root). The experimental IgA nephropathy model was established by the immunity combination method of oral BSA and injection of SEB. It was found that combination of LC/MS technology with PCA can be successfully applied to phospholipids profile analysis, clearly classify the model group and normal group, and PA-MSHA treatment group is closer to the normal control group than medicine control group. The result showed that Th2/Th1 (=CD4+CD30+/CD4+CD30−) of the model group is 20.70 ± 3.57, which is significantly higher than that of the control group (1.34 ± 0.14) (P < 0.001). The Th2/Th1 ratio of the PA-MSHA treatment group and the medicine control group are lower than that of the model group (P < 0.01). It is suggested that mouse IgA nephropathy has the phospholipids metabolic abnormality, PA-MSHA vaccine cannot only regulate the abnormal phospholipids metabolism mouse with the IgA nephropathy, but also correct the over unbalance of Th2/Th1 proportion.