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Optimization of culture conditions for the production of thermostable polygalacturonase by Penicillium SPC-F 20

2008; Springer Science+Business Media; Volume: 35; Issue: 9 Linguagem: Inglês

10.1007/s10295-008-0375-0

1476-5535

Abraham Mathew, Abi N. Eldo, A. G. Molly,

Enzyme Production and Characterization

Penicillium strain isolated from citrus fruit was found to produce thermostable polygalacturonases. Optimization of process parameters resulted in high levels of enzyme production after 3 days of incubation at a pH of 5.0 at 30 degrees C in the presence of 1% pectin. The optimum temperature for enzyme activity was 60 degrees C and a pH of 5.5 was found to be the optimal pH. The enzyme showed a high level of thermostability in the presence of substrate with a residual activity of 48% after 2 h of incubation at 60 degrees C. A thermostable nature with a high pH range for activity makes it an industrially important enzyme.