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Establishing of a Method Combined Immunomagnetic Separation with Colloidal Gold Lateral Flow Assay and Its Application in Rapid Detection of Escherichia coli O157:H7

2013; Elsevier BV; Volume: 41; Issue: 12 Linguagem: Inglês

10.1016/s1872-2040(13)60696-9

0253-3820

Cui Xi, Qirong Xiong, Yonghua Xiong, Shan Shan, Weihua Lai,

Escherichia coli research studies

In this study, immunomagnetic beads were prepared by coupling anti- Escherichia. coli O157:H7 monoclonal antibodies with magnetic beads. A colloidal gold test strip was composited with anti- E. coli O157:H7 monoclonal antibody marked by colloidal gold as the detection antibody, anti-E. coli O157:H7 polyclonal antibody as the test line, and donkey anti-mouse IgG secondary antibody as the control line. Immunomagnetic separation was coupled with colloidal gold lateral flow assay to detect E. coli O157:H7 rapidly. Results showed that 1 mL 7.6 × 103 colony-forming unit (CFU) mL1 E. coli O157:H7 exhibited a positive outcome when colloidal gold lateral flow assay was performed after immunomagnetic separation. A food sample inoculated with 10 CFU/g E. coli O157:H7 after 6 h of enrichment could be detected within 72 min of immunomagnetic separation and colloidal gold lateral flow assay. The method proposed in this study could be used to on-site detect harmful substances in food significantly and rapidly.