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The kinetics of the killing of mouse tumour cells in vivo by immune responses

1972; Wiley; Volume: 10; Issue: 1 Linguagem: Inglês

10.1002/ijc.2910100115

1097-0215

D. D. Porteous, T. R. Munro,

Immunotherapy and Immune Responses

Abstract Immune responses against the C3H mouse ascites tumour BP8 were studied in vivo by measuring the proportion of an inoculum that was killed in allogeneic C57BL animals. Killing was assayed by labelling the cells with 125 IUdR; the 125 I liberated from dead cells was excreted, and the proportion of label (and thus the proportion of cells) remaining was measured by whole‐body counting. Cell loss from the primary immune response began about a week after intraperitoneal injection of 10 7 cells, and was accelerated by addition of 10 7 ‐10 8 heavily irradiated cells to the inoculum. By contrast, it began immediately in immunized mice. Labelled cells irradiated with 10,000 R of X‐rays did not begin to lose 125 I until 2 days after irradiation, and so could be used to investigate the immune status of an animal without fear of killing it.