Molecular cloning and characterization of FHL2, a novel LIM domain protein preferentially expressed in human heart
1998; Elsevier BV; Volume: 210; Issue: 2 Linguagem: Inglês
10.1016/s0378-1119(97)00644-6
ISSN1879-0038
AutoresKwok Keung Chan, Stephen Kwok‐Wing Tsui, Simon Ming‐Yuen Lee, Sharon Chui Wah Luk, Choong Chin Liew, Kwok‐Pui Fung, Mary Miu Yee Waye, Cheuk Yu Lee,
Tópico(s)Viral Infections and Immunology Research
ResumoA full-length cDNA clone encoding a novel LIM-only protein was isolated and sequenced from a human fetal heart cDNA library. This full-length clone consists of 1416 base pairs and has a predicted open reading frame (ORF) encoding 279 amino acids. The ORF of this polypeptide codes for the human heart-specific four and a half LIM-only protein 2 (FHL2). It possesses an extra zinc finger that is a half LIM domain and four repeats of LIM domain. When the human FHL2 cDNA probe was used to hybridize with poly-A RNA of various human tissues, a very strong signal could be seen in heart tissues, and only moderately low signals could be detected in placenta, skeletal muscle and ovary. Virtually no signal could be detected in brain, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon or peripheral blood leukocyte. FHL2 was mapped to chromosome 2q12–q13 by fluorescent in-situ hybridization (FISH).
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