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AFM Nanodissection Reveals Internal Structural Details of Single Collagen Fibrils

2003; American Chemical Society; Volume: 4; Issue: 1 Linguagem: Inglês

10.1021/nl034685n

1530-6992

Chuck K. Wen, M. Cynthia Goh,

Cellular Mechanics and Interactions

We utilize the atomic force microscope (AFM) to perform a careful nanodissection procedure on a single collagen fibril. By precisely incising the fibril and then peeling away exterior layers, its internal structure was successfully exposed for direct visualization. This methodology has the advantage of avoiding unnecessary rough contact with the specific area being examined as well as high lateral frictional forces, both of which can hinder attempts to dissect delicate biological specimens with the AFM.