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Characterization of an antihypertensive peptide from an Alfalfa white protein hydrolysate produced by a continuous enzymatic membrane reactor

2006; Elsevier BV; Volume: 41; Issue: 9 Linguagem: Inglês

10.1016/j.procbio.2006.04.019

1873-3298

Romain Kapel, Elhassan Rahhou, Didier Lecouturier, Didier Guillochon, Pascal Dhulster,

Meat and Animal Product Quality

An industrial Alfalfa white protein concentrate hydrolysed at pilot plant scale by Delvolase® in a continuous enzymatic membrane reactor leads to a hydrolysate exhibiting a high ACE inhibition activity (8.8 μg/ml). This hydrolysate, fractionated by two chromatography steps (exclusion size fast protein liquid chromatography and reversed-phase high performance liquid chromatography) revealed that around 15% of the total ACE inhibition activity is due to a single ACE inhibitory peptide. This peptide identified by electrospray mass spectrometry as VW, a potent antihypertensive peptide in vivo, contained four times of that in the main Alfalfa white protein (RuBisCO).