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Effects of vanadate on the oxidation of NADH by xanthine oxidase

1989; Elsevier BV; Volume: 269; Issue: 1 Linguagem: Inglês

10.1016/0003-9861(89)90099-4

1096-0384

Stefan I. Liochev, E Ivancheva, Irwin Fridovich,

Paraquat toxicity studies and treatments

Vanadate (V(v)) stimulates the oxidation of NADH by xanthine oxidase and superoxide dismutase eliminates the effect of V(v). Paraquat stimulates both the oxidation of NADH by xanthine oxidase and the V(v) enhancement of that oxidation. Xanthine, which is a better substrate for xanthine oxidase than is NADH, causes a V(v)-dependent cooxidation of NADH which is transient and eliminated by SOD. Urate inhibits the V(v)-stimulated oxidation of NADH by xanthine oxidase or by Rose Bengal plus light. Measurement of rates of both O2− production and V(v)-stimulated NADH oxidation showed that many molecules of NADH were oxidized per O2−. These chain lengths were an inverse function of overall reaction rate. Minimum chain lengths, calculated on the basis of 100% univalent reduction of O2 to O2−, were smaller than measured average chain lengths by a factor of five. All of these results are in accord with the view that V(v) does not directly affect the activity of the enzyme, but rather catalyzes the free radical chain oxidation of NADH by O2−. It was further shown that phosphate was not involved and that the active form of V(v) was orthovanadate, rather than decavanadate.