Produção Nacional
Revisado por pares
Real-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia
2007; Elsevier BV; Volume: 116; Issue: 4 Linguagem: Inglês
10.1016/j.exppara.2007.02.011
ISSN1090-2449
AutoresBianca Ervatti Gama, Felipe do Espirito Santo Silva-Pires, Mauro N’Kruman R. Lopes, Maria Angélica Cardoso, Constança Britto, Kátia Luz Torres, Leila de Mendonça Lima, José Maria de Souza, Cláudio Tadeu Daniel‐Ribeiro, Maria de Fátima Ferreira‐da‐Cruz,
Tópico(s)Vector-borne infectious diseases
ResumoWe have optimized a faster and cheaper real-time PCR and developed a conventional genus specific PCR based on 18S rRNA gene to detect malaria parasites in low-grade parasitemias. Additionally, we compared these PCRs to the OptiMAL-IT™ test. Since there is no consensus on choice of standard quantitative curve in real-time assays, we decided to investigate the performance of parasite DNA from three different sources: “genome”, amplicon and plasmid. The amplicon curve showed the best efficiency in quantifying parasites. Both PCR assays detected 100% of the clinical samples tested; the sensitivity threshold was 0.5 parasite/μl and no PCR positive reaction occurred when malaria parasites were not present. Conversely, if OptiMAL-IT™ were employed for malaria diagnosis, 30% of false-negative results could be expected. We conclude that PCR assays have potential for detecting malaria parasites in asymptomatic infections, in evaluation of malaria vaccine molecule candidates, for screening blood donors, especially in endemic areas, or even in monitoring malaria therapy.
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