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The Effects of the Diterpenes Isolated from the Brazilian Brown Algae Dictyota pfaffii and Dictyota menstrualis against the Herpes Simplex Type-1 Replicative Cycle

2009; Thieme Medical Publishers (Germany); Volume: 76; Issue: 04 Linguagem: Inglês

10.1055/s-0029-1186144

1439-0221

Juliana L. Abrantes, Jussara P. Barbosa, Diana Negrão Cavalcanti, Renato Piai Pereira, Carlos Frederico Leite Fontes, Valéria Laneuville Teixeira, Thiago Moreno L. Souza, Izabel Christina Nunes de Palmer Paixão,

Echinoderm biology and ecology

We describe in this paper that the diterpenes 8,10,18-trihydroxy-2,6-dolabelladiene (1) and (6R)-6-hydroxydichotoma-4,14-diene-1,17-dial (2), isolated from the marine algae Dictyota pfaffii and D. menstrualis, respectively, inhibited HSV-1 infection in Vero cells. We initially observed that compounds 1 and 2 inhibited HSV-1 replication in a dose-dependent manner, resulting in EC50 values of 5.10 and 5.90 µM, respectively, for a multiplicity of infection (MOI) of 5. Moreover, the concentration required to inhibit HSV-1 replication was not cytotoxic, resulting in good selective index (SI) values. Next, we found that compound 1 sustained its anti-herpetic activity even when added to HSV-1-infected cells at 6 h after infection, while compound 2 sustained its activity for up to 3 h after infection, suggesting that these compounds inhibit initial events during HSV-1 replication. We also observed that both compounds were incapable of impairing HSV-1 adsorption and penetration. In addition, the tested molecules could decrease the contents of some HSV-1 early proteins, such as UL-8, RL-1, UL-12, UL-30 and UL-9. Our results suggest that the structures of compounds 1 and 2, Brazilian brown algae diterpenes, might be promising for future antiviral design.