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Identification and Isolation of Foot-and-Mouth Disease Virus from Primary Suspect Cases in Korea in 2000.

2003; Japanese Society of Veterinary Science; Volume: 65; Issue: 1 Linguagem: Inglês

10.1292/jvms.65.1

1347-7439

Jinho Shin, Hyun‐Joo Sohn, Kang-Seuk Choi, Byung-Jun Kwon, Cheong-Up Choi, Jae-Hoon Kim, Eui-Kyung Hwang, Jong-Hyeon Park, Jong-Yeom KIM, Sang Ho Choi, Ok‐Kyung Kim,

Viral Infections and Immunology Research

The Republic of Korea had been free from foot and mouth disease (FMD) since 1934, until a recent outbreak in 2000. From March to April 2000, a total of 15 FMD outbreaks due to the serotype O virus were recorded. Coincidental outbreaks of FMD in cattle or pigs by the serotype O virus were reported in the region, including Taiwan, China, Japan, Russia and Mongolia. In this report, the results of emergency investigations of FMD cases on a dairy farm located approximately 5-km from the demilitarized zone in Korea are described. The causative agent of the disease was identified as the FMD virus O by reverse transcription-polymerase chain reaction (RT-PCR) assays using primers derived from the 3D polymerase, internal ribosome entry site (IRES), 1D/2B regions, enzyme-linked immunosorbent assay (ELISA) for antigen detection and typing. Sequence data of the partial 1D/2B region obtained from vesicular fluid showed close similarity (98% sequence identity) to the Kinmen isolate of the FMD virus O in Taiwan. The causative virus was isolated using black goat fetal lung cells following propagation in unweaned mice.