Separation of aldehyde reductases and alcohol dehydrogenase from brain by affinity chromatography: Metabolism of succinic semialdehyde and ethanol
1975; Elsevier BV; Volume: 63; Issue: 4 Linguagem: Inglês
10.1016/0006-291x(75)90662-2
ISSN1090-2104
AutoresBoris Tabakoff, Jean P. von Warburg,
Tópico(s)Alcohol Consumption and Health Effects
ResumoEnzyme activities which reduced aldehydes such as succinic semialdehyde in rat brain cytosol were separated by affinity chromatography on NADP-Sepharose. The reduction of succinic semialdehyde which was previously thought to be catalyzed by alcohol dehydrogenase or lactate dehydrogenase was shown to be primarily catalyzed by enzymes both separable from and characteristically different from these enzyme activities. Brain alcohol dehydrogenase activity was shown to be <1mU/gm brain. Brain activity reducing succinic semialdehyde to γ-hydroxybutyrate, a known hypnotic, was also shown to differ from previously described aldehyde reductases.
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