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ALLOTYPING FOR HLA CLASS I USING PLASMA AS ANTIGEN SOURCE

1989; Wiley; Volume: 16; Issue: 2 Linguagem: Inglês

10.1111/j.1744-313x.1989.tb00457.x

1744-313X

H. Grosse‐Wilde, Ilias I.N. Doxiadis,

T-cell and B-cell Immunology

Immunoadsorption of soluble HLA class I antigens onto immunobeads, one-dimensional iso-electric focusing of these proteins and subsequent immunoblotting allows a biochemical identification of HLA class I allotypes. The distinct protein bands can be clearly attributed to particular HLA antigens and are comparable to those observed after detergent solubilization of membrane-bound HLA antigens. Segregation analysis showed that the biochemically detected pattern of soluble class I gene products followed Mendelian inheritance. However, antigens such as HLA-A1, -A2, -B8, and -B51 were not always clearly detectable, a phenomenon attributable to either different plasma concentrations of these HLA antigens or variable affinity of the monoclonal antibody used to capture class I antigens. These results show that in principle allotyping of HLA class I using plasma as the antigen source is feasible, but with the limitation that some antigens may not be easily detected in some individuals.