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Association of Maternal Histocompatibility at Class II HLA Loci with Maternal Microchimerism in the Fetus

2004; Springer Nature; Volume: 56; Issue: 1 Linguagem: Inglês

10.1203/01.pdr.0000129656.10005.a6

1530-0447

Stanley M. Berry, Sonia S. Hassan, Evelyne Russell, Debra Kukuruga, Susan Land, Joseph Kaplan,

Assisted Reproductive Technology and Twin Pregnancy

For investigating the possible influence of maternal-fetal HLA compatibility on maternal microchimerism, DNA samples from blood of 120 maternal-fetal pairs were genotyped at two polymorphic loci: glutathione-S-transferase M1 (GSTM1) and angiotensin-converting enzyme (ACE). Informative pairs (mother heterozygous/fetus homozygous at one of the two loci) were then tested by quantitative real-time PCR for the noninherited maternal allele(s) and genotyped at the HLA-A, B, and C class I loci and/or at the DRB1 and/or DQB1 class II loci. Small numbers of maternal cells were detected in the circulation of 16 of the 30 informative second- and third-trimester fetuses. Comparison with HLA data suggested an association between microchimerism and maternal compatibility at the class II DRB1 and/or DQB1 HLA loci and with the maternal HLA-DQB1*0301 allele. There was no relationship between maternal microchimerism and maternal-fetal HLA compatibility at other HLA loci or with gestational age, fetal anomalies, or red cell or platelet isoimmunity.