Development of a Specific Enzyme-Linked Immunosorbent Assay (ELISA) for the Analysis of the Organophosphorous Pesticide Fenthion in Real Samples Based on Monoclonal Antibody
2011; Taylor & Francis; Volume: 44; Issue: 9 Linguagem: Inglês
10.1080/00032719.2010.520391
ISSN1532-236X
AutoresLimin Wang, Qi Zhang, Defeng Chen, Yang Liu, Chuanyong Li, Baishi Hu, Dan Du, Fengquan Liu,
Tópico(s)Protein purification and stability
ResumoA new specific monoclonal antibody against the organophosphorous pesticide fenthion was produced based on our previous study. A sensitive and specific Enzyme-Linked Immunosorbent Assay (ELISA) for fenthion based on the new immunizing/coating hapten combination was developed. In this study, the H1 which attempts to expose the aromatic ring group was conjugated with bovine serum albumin (BSA) for the immunogen. All of the haptens that have been synthesized were conjugated with ovalbumin (OVA) for the coating antigen. The efficient antibody/coating conjugated combinations were selected, and the optimized ELISA was developed. In the optimized system, the IC50 value was 5.8 ng · mL−1 with the detection limit (IC20) of 0.028 ng · mL−1. The cross reactivity with all of the tested pesticides was lower than the 0.5%. Also, the system can detect the fenthion addition to the real samples such as soil, water, rice, and Chinese cabbages.
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