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Phylogenetic relationships in Solanaceae and related species based on cpDNA sequence from plastid trnE-trnT region

2008; Brazilian Society of Plant Breeding; Volume: 8; Issue: 1 Linguagem: Inglês

10.12702/1984-7033.v08n01a12

1984-7033

Danila Montewka Melotto‐Passarin, Irving J. Berger, K. Dressano, Virginia Martín, Giancarlo Conde Xavier Oliveira, Ralph Bock, Helaine Carrer,

Plant and Fungal Species Descriptions

1 Laboratorio de Biotecnologia Agricola, Departamento de Ciencias Biologicas, Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), Universidade de Sao Paulo (USP), Avenida Padua Dias, 11, 13.418-900, Piracicaba, SP, Brasil. *E-mail: hecarrer@esalq.usp.br 2 Companhia de Cigarros Souza Cruz, Av. Gal Pinto Tourinho, 3200, 83.880-000, Bom Jesus, Rio Negro, PR, Brasil 3 Departamento de Genetica, ESALQ, USP 4 Max Planck Institute of Molecular Plant Physiology, Am Muhlenberg 1, D-14476, Potsdam, Golm, Germany ABSTRACT Intergenic spacers of chloroplast DNA (cpDNA) are very useful in phylogenetic and population genetic studies of plant species, to study their potential integration in phylogenetic analysis. The non-coding trnE-trnT intergenic spacer of cpDNA was analyzed to assess the nucleotide sequence polymorphism of 16 Solanaceae species and to estimate its ability to contribute to the resolution of phylogenetic studies of this group. Multiple alignments of DNA sequences of trnE-trnT intergenic spacer made the identification of nucleotide variability in this region possible and the phylogeny was estimated by maximum parsimony and rooted with Convolvulaceae Ipomoea batatas, the most closely related family. Besides, this intergenic spacer was tested for the phylogenetic ability to differentiate taxonomic levels. For this purpose, species from four other families were analyzed and compared with Solanaceae species. Results confirmed polymorphism in the trnE-trnT region at different taxonomic levels.