AF2, an Ascaris neuropeptide: Isolation, sequence, and bioactivity
1993; Elsevier BV; Volume: 14; Issue: 3 Linguagem: Inglês
10.1016/0196-9781(93)90127-3
ISSN1873-5169
AutoresCynthia Cowden, Antony O.W. Stretton,
Tópico(s)Nematode management and characterization studies
ResumoA FMRFamide-like neuropeptide, KHEYLRFamide (Lys-His-Glu-Tyr-Leu-Arg-Phe-amide; AF2), was isolated from a head extract of the nematode Ascaris suum by using a three-step HPLC separation. In a dorsal muscle strip preparation, synthetic AF2 produced multiple effects on muscle tension: a slow relaxation was followed by contraction and rhythmic activity. Sulfated AF2 was no more potent than AF2. The effects on muscle tension were correlated with electrical activity recorded intracellularly from muscle cells. AF2 markedly increased the tension change associated with changes in muscle membrane potential.
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