Biosynthesis of Poly(3-hydroxybutyrate- c o -3-mercaptobutyrate) as a Sulfur Analogue to Poly(3-hydroxybutyrate) (PHB)
2001; American Chemical Society; Volume: 2; Issue: 3 Linguagem: Inglês
10.1021/bm015564p
ISSN1526-4602
AutoresTina Lütke‐Eversloh, Klaus Bergander, Heinrich Luftmann, Alexander Steinbüchel,
Tópico(s)Microplastics and Plastic Pollution
ResumoA hitherto unknown copolymer that contains sulfur in the backbone linking 3-hydroxybutyrate and 3-mercaptobutyrate by thioester linkages, poly(3HB-co-3MB), was synthesized by the polyhydroxyalkanoate-(PHA-) accumulating bacterium Ralstonia eutropha, when 3-mercaptobutyric acid was fed as the carbon source in addition to gluconate. The chemical structure of this novel polymer was confirmed by gas chromatography/mass spectrometry, infrared spectroscopy, 1H and 13C nuclear magnetic resonance spectroscopy, and elemental sulfur analysis. Different poly(3HB-co-3MB) samples were isolated and characterized, and the molar 3MB fraction contributed up to 72 mol%. Regarding the chemical structure of 3-mercaptobutyrate, which is analogous to 3-hydroxybutyrate, the incorporation of this novel polymer constituent represents a second example of 3-mercaptoalkanoates that are incorporated into biopolymers by the PHA synthase of R. eutropha.
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